Database for Annotation, Visualization and Integrated Discovery 2.1
National Institute of Allergy and Infectious Disease
The Database for Annotation, Visualization and Integrated Discovery
DAVID Functional Annotation Table
Gene Report
Help and Manual

Right-click and select 'Save Target As' to download results Download File
twist family bHLH transcription factor 1(TWIST1) twist family bHLH transcription factor 1(TWIST1) Related Genes Homo sapiens
CYTOBAND 7p21.2,
GENERIF_SUMMARY Twist1 and Twist2 antagonize the ARF/p53 pathway. This is the first report suggesting a role for Twist genes as oncogenes, new TWIST mutation (7p21) with variable eyelid manifestations supports locus homogeneity of BPES at 3q22, Deletion of 18 nucleotides or insertion of three, 15, or 21 nucleotides was not consistently associated with clinical disease and was conclude that they are at most weakly pathogenic., Identified direct paternal transmission of a novel missense TWIST mutation in the highly conserved Helix II domain of this bHLH-family gene., Deficience causes skull osteoblast apoptosis due to increased TNFalpha expression and caspase-2 activation., Cooperative E-box regulation of human GLI1 by this protein and USF, Characterization of a dominant negative C. elegans Twist mutant protein with implications for human Saethre-Chotzen syndrome, inactivation reduces CBFA1/RUNX2 expression and DNA binding to the osteocalcin promoter in osteoblasts, One variant is associated with Robinow-Sorauf syndrome., The risk for developmental delay in patients with deletions involving the TWIST gene is approximately 90%., novel role for TWIST, a basic helix-loop-helix protein, which plays a central role in cell type determination and differentiation, during generation of acquired resistance to taxol in a nasopharyngeal carcinoma cell line, HNE1-T3, TWIST1 plays an essential role in tumor metastasis., Twist was highly and selectively expressed in T cells of patients with Sezary Syndrome., loss of HOXA5 expression could lead to the functional activation of Twist resulting in aberrant cell cycle regulation and promoting breast carcinogenesis, A reporter assay with the p21 promoter demonstrated that Snail inhibited expression of p21 induced by E2A. Co-expression of Snail with Twist showed additive inhibitory effects., H-Twist overexpression in neuroblastomas is responsible for the inhibition of the ARF/p53 pathway involved in the Myc-dependent apoptotic response., Misregulation of Twist1 dimerization by post-translational mechanisms underlies phenotypes of individuals with Saethre-Chotzen syndrome, This analysis further dissects the structure-function relationships of TWIST and corroborates with phenotypic observations of disease expressivity., TWIST gene is involved in high-grade pediatric osteosarcomas and is a new marker with a possible initial predictive value., Results have identified TWIST as a critical regulator of prostate cancer cell growth and suggest a potential therapeutic approach to inhibit the growth and metastasis of androgen-independent prostate cancer through inactivation of the TWIST gene., Overexpression of TWIST protein in a human glioma cell line significantly enhanced tumor cell invasion, a hallmark of high-grade gliomas., Twist1 is induced by canonical Wnt signaling and expression of Twist1 strongly inhibited chondrocyte gene expression., Twist over-expression induces angiogenesis in a mouse breast cancer model, Twist may play an important role in the invasion and metastasis of nasopharyngeal carcinoma, Dimer partner selection is an important mediator of Twist1 function in transgenic mice, providing a mechanistic understanding of craniosynostosis due to TWIST haploinsufficiency., This provides genetic evidence that Twist1, Msx2 and Efna4 function together in boundary formation and the pathogenesis of coronal synostosis., Twist overexpression plays a role in destabilizing the genome, thus promoting chromosomal instability., These results implicate Twist proteins in regulation of TNFalpha production by antiinflammatory factors and pathways, and provide a mechanism by which type I IFNs and Axl receptors suppress inflammatory cytokine production., Twist may act as a negative regulator of osteoblastic differentiation in periodontal ligament cells, Twist haploinsufficiency results in decreased Cbl-mediated PI3K degradation in osteoblasts., Patients with TWIST gene mutations may have more ophthalmic abnormalities, including more strabismus, ptosis, NLDO, astigmatism, vertical deviations, and amblyopia compared with patients with FGFR3 gene mutations., suggest critical implication of the CCT repeats in association with Sp1 and Sp3 factors in sustaining expression of the TWIST1 gene in mesenchymal cells, Twist, a novel oncogene, is upregulated in pancreatic cancer, Induction of Twist by a human viral oncoprotein LMP1 directly contributes to the metastatic nature of nasopharyngeal carcinoma., Twist is a positive transcriptional regulator of AKT2 expression;Twist-AKT2 signaling is involved in promoting invasive ability and survival of breast cancer cells., Some TWIST1 mutations in the RUNX2 binding site are associated with craniosynostosis., TWIST may serve as a prognostic marker for high-grade prostatic cancer in humans; up-regulation of TWIST with aberrant expression of E-cadherin may predict development of metastatic disease., Data suggest that Twist-1 and -2 play an important role in NF-kappaB-dependent chemoresistance., no evidence that mosaicism for mutations, normally associated with syndromal forms of craniosynostosis, occur in single suture craniosynostosis, findings suggest that reduced expression of TWIST suppresses the multistep process of peritoneal dissemination (detachment from the primary lesion, adhesion to MCs and invasion of MCs., Twist regulates cell motility and invasion in gastric cancer cell lines, probably through the N-cadherin and fibronectin production, TWIST plays a key role in the continuous proliferation of immortalized cells. Over-expression of TWIST results in down-regulation of p14(ARF), which leads to the impairment of DNA damage checkpoint in response to genotoxic stress., TWIST1 was frequently observed in the bone marrow of breast cancer patients before chemotherapy; expression of TWIST1 correlated with the rapid occurrence of distant metastasis or local progression, within 1 year from initial diagnosis, TWIST may be a useful prognostic marker and target for gastric cancer therapy., analysis of transcriptional activation by the proto-oncogene Twist1, Twist was found in cell membrane and cytoplasm of cervical cancers.High expression predicted poor survival; it was a significant prognostic factor in radiation-sensitive squamous cell carcinomas but not in radiation-resistant adenocarcinomas., Results identified TWIST1 loci showing significant differential DNA methylation levels between tumor and non-tumor lung and highly significant hypermethylation in adenocarcinoma., Twist may play an important role in the angiogenesis and metastasis of hepatocellular carcinoma., Using transient promoter assays, we show that Twist can down-regulate E-cadherin promoter activity by up to two folds., Both YB-1 and Twist expression could induce tumor progression, promoting cell growth and driving oncogenesis in various cancers., Twist was up-regulated in primary liver cancer., TWIST microdeletion is associated with Saethre-Chotzen phenotype and a complex rearrangement involving chromosomes 2 and 7, a key signalling pathway involving HIF-1alpha and TWIST promotes metastasis in response to intratumoural hypoxia, These data indicate that MSX2 plays a crucial role in pancreatic cancer development by inducing changes consistent with epithelial to mesenchymal transition through enhanced expression of Twist 1., STAT3, Twist, and AKT2 form a functional signaling axis to regulate pivotal oncogenic properties of cancer cells, summary of the effects of FGFR2 and Twist genetic mutations resulting in altered osteoblast phenotype and premature cranial fusion based on analysis in human syndromic craniosynostosis [review], Positive Twist expression may be a useful prognostic marker for patients with bladder cancer, and its expression seems to be correlated to the tobacco status of the patients., high expression level of Twist is closely associated with more aggressive behaviors of human hepatocellular carcinoma, p53 interacted with the N-terminal domain of Twist and repressed Twist-dependent YB-1 promoter activity., thrombin up-regulates Twist, which is required for thrombin-induced angiogenesis as measured by endothelial cell migration, Matrigel tubule formation, and tumor angiogenesis, Twist proteins promote malignant conversion and metastatic dissemination., Th lymphocytes from the inflamed joint or gut tissue of patients with rheumatic diseases, Crohn's disease or ulcerative colitis expressed high levels of twist1., In human melanoma cells, MFG-E8 knockdown attenuated Akt and Twist signaling and thereby compromised tumor cell survival, EMT, and invasive ability, TWIST regulates the expression of several genes involved in the differentiation, adhesion, and proliferation of gastric cancer cells., TWIST1 promoter methylation is significantly more prevalent in malignant compared with healthy breast tissue., using gene expression profiling, several genes differentially expressed in human choroid plexus papillomas could be identified. TWIST1 is highly expressed in choroid plexus papillomas and promotes proliferation and invasion., The nuclear receptor PPARdelta not only mediates the actions of PGC-1alpha but also regulates twist-1 expression, suggesting a negative-feedback regulatory mechanism., Studied TWIST1 mutation confirmed Saethre-Chotzen syndrome patients and possible increased risk of cancer. Found breast cancer risk not increased in individuals with TWIST1 mutation confirmed Saethre-Chotzen syndrome., We found upregulation of mRNA for transcription factors Snail, Slug, Twist, and SIP1 in spindle cell carcinoma when compared to squamous cell carcinoma., We describe for the first time that EMT markers Snail and Twist are expressed in PCC and that their expression is associated with malignancy, Results show that cumulative RASSF1A, TWIST1, and HIN1 gene methylation accurately distinguished ducts with cancerous versus benign lesions., Negative regulation of TIMP1 is mediated by transcription factor TWIST1., Results demonstrates the presence of two putative putative nuclear localization signals (NLS) motifs in H-TWIST and suggests that these NLS sequences are functional., results suggest that TWIST1 may be a useful genetic marker for osteoporosis and may have a role on bone metabolism in humans., These findings implicate the TWIST gene family members as potential mediators of MSC self-renewal and lineage commitment in postnatal skeletal tissues by exerting their effects on genes involved in the early stages of bone development., hypoxic TWIST1 induction is regulated by both HIF-1 and HIF-2 proteins through distinct regulatory elements in a tissue-specific manner, Data suggest that modulation of castration-induced oxidative stress or Twist1/AR signaling might be a useful strategy for developing a novel therapeutics in prostate cancer., upregulated Twist expression in the primary ESCC significantly correlates with the high risk of subsequent development of distant metastasis and low postoperative survival rates., Coexpression of Snail and Twist correlated with the worst prognosis of hepatocellular carcinoma., Twist contributes to EMT in the model of virus-induced pulmonary fibrosis, Girl with clinical features of Saethre-Chotzen syndrome who has a previously undescribed sequence variant in the TWIST1 gene, corresponding to p.R191M., We have analysed women with BRCA1/2-negative hereditary breast cancer to study whether these families might have mutations in Saethre-Chotzen-associated genes., Twist1 represents one of the factors that promotes pathogenic accumulation of fibroblasts in fibrotic lung disease., Down-regulation of Twist1 could increase drug sensitivity of nasopharyngeal carcinoma cell line HNE1 to taxol by inducing apoptosis., Twist1 may play a role in inflammation of human white adipocytes because it can regulate the expression and secretion of inflammatory adipokines via direct transcriptional effects in white adipocytes, Data demonstrate that the overexpression of Twist in breast cells can promote the generation of a cancer stem cell phenotype characterized by the high expression of CD44, little or no expression of CD24, and increased aldehyde dehydrogenase 1 activity., Twist promotes breast cancer stem cell formation via down-regulation of CD24, Evaluation of Twist and E-cadherin expressions should be useful for determining tumor properties, including prognosis, in patients with esophageal squamous cell carcinoma, TWIST1 may play a pivotal role in paclitaxel-induced apoptosis of Hep-2 cells., The TWIST1 methylation level was higher in colorectal adenoma and cancer than in normal colorectal mucosa. Elevated TWIST1 mRNA expression in normal colorectal mucosa in patients with CRC as well as in primary CRC specimens was assoc'd with poor outcomes., Findings indicate that Twist1 induces HCC invasion via increased activity in MMPs, specifically in MMP2 and MMP9, leading to poor clinical prognoses., The positive expression of Twist was correlated to lymph node metastasis in invasive lobular carcinoma., Twist1 as a regulator of miRNA cluster responsible for the regulation of the IKKbeta/NF-kappaB and PTEN/AKT pathways and its association of ovarian cancer stem cell differentiation, PKB phosphorylates one of the anti-apoptotic proteins--transcription factor Twist-1 at Ser42., HIN-1, RAR-beta, RASSF1A, and Twist had the ability to distinguish high-grade squamous intraepithelial lesions/squamous cell carcinomas from low-grade squamous intraepithelial lesions/negative cervical scrapings, The methylation levels and frequencies of HIN1, RASSF1A and TWIST, and average MR were significantly lower in basal-like subtype compared to luminal or human epithelial growth factor receptor-2 subtype., role for dysregulation of TWIST in the pathophysiology of myelodysplastic syndromes, Studies indicate that understanding of the mechanistic insights in the role of Twist in lineage specification/differentiation and tumorigenesis is vital to the success of future clinical applications for the therapeutic use of MSCs., studies demonstrated that TWIST1 enhances glioblastoma multiforma invasion in concert with mesenchymal change not involving the canonical cadherin switch of carcinoma epithelial-mesenchymal transition, This article summarizes the recent knowledge on the role and mechanisms of action of Twist1 in the control of skeletal cell fate with specific emphasize on postnatal bone biology and pathology., These data implicate Twist1 as a critical regulator of valve development and suggest that Twist1 influences extracellular matrix production and cell proliferation during disease., The results suggest that Twist1-induced epithelial-mesenchymal transition and tumour-initiating capability in cancer cells occurs through chromatin remodelling, which leads to unfavourable clinical outcomes., ILK regulates the expression of Her2/neu through TWIST and YB-1, lending support to the use of ILK inhibitors in the treatment of aggressive Her2/neu-positive tumors., Thrombin upregulates Twist via HIF-1alpha to make tumor cells malignant and also establish a link between the coagulation disorder and cancer metastasis., Results showed the prognostic relevance of bone marrow disseminated tumor cells assessed by a multimarker mRNA panel consisting of TWIST1, cytokeratin 19 (gene name, Low twist1 expression in human white adipose tissue correlates with obesity and an insulin-resistant phenotype, which may be mediated by an increased sensitivity to the proinflammatory effect of TNFalpha., Our data indicate that deregulation of TWIST is somehow important in the aggressiveness of triple-negative carcinomas and poor patient outcome, The present study showed that Twist1 regulates YB-1 expression and that both Twist1 and YB-1 promote malignant potentials, including tumour growth, invasion and anti-cancer-drug resistance in bladder cancer cells, Deregulation of TWIST-1 in the CD34+ compartment is associated with chronic myeloid leukemia., Foxo3a suppression of urothelial cancer invasiveness through Twist1, Y-box-binding protein 1, and E-cadherin regulation., results show that Twist1 needs to induce Snail2 to suppress the epithelial branch of the EMT program and that Twist1 and Snail2 act together to promote EMT and tumor metastasis, Twist1 is related to metastasis by tumor cells un hepatocellular carcinoma angiogenesis., activated STAT3 signal may associate with Twist and E-cadherin expression and mediate HCC invasiveness and metastasis., The results indicate that Snail1 controls Zeb1 expression at multiple levels and acts cooperatively with Twist in the ZEB1 gene transcription induction., Expression of transcriptional factors zeb1 and twist mainly occur in the stromal compartment of breast carcinomas., Data show that three patients carrying small deletions containing TWIST1 who present with significant postnatal microcephaly, facial dysmorphism, and short stature., TWIST1 plays a key role in determining malignant features of the anaplastic phenotype in vitro, Data show that induction of PDGFRalpha and invadopodia is essential for Twist1 to promote tumor metastasis., TWIST1 hypermethylation was detected in colorectal specimens of 46 patients with cancer, but in none of the tissues from the nonmalignant control group., TWIST1 expression levels could be a tumor indicator in stage II patients., Loss of p27(Kip)(1) causes an elongated/scatter cell-like phenotype involving up-regulation of Brachyury and Twist gene expression., Twist1, a basic helix-loop-helix transcription factor, promotes breast tumor cell epithelial-mesenchymal transition (EMT), invasiveness, and metastasis., the TWIST mutation (R154S) may be an essential underlying factor in the development and pathophysiology of giant cell tumor of bone, These results suggest that the overexpression of Twist correlates to hypoxia-induced vasculogenic mimicry in hepatocellular carcinoma cells., Twist expression can be regulated at the post-translational level through phosphorylation by CK2, which increases Twist stability in response to IL-6 stimulation, Phosphorylation of the Twist1-family basic helix-loop-helix transcription factors is involved in pathological cardiac remodeling and can be regulated by Akt signaling, analysis of twist expression in lung cancer provide clues to comprehensive understanding of the mechanisms, by which metastasis often develops in lung cancer, Twist1 overexpression in DPSCs enhanced expression of DSPP, gene that marks odontoblast terminal differentiation. Twist1 stimulates Dspp promoter activity by antagonizing Runx2 function in 293FT cells., High TWIST1 is associated with esophageal squamous cell carcinoma and associated with its epithelial-mesenchymal transition., Twist expression correlates with lymph node metastasis. The mechanism involved in such a correlation may be related to lymphangiogenesis., We report functional and structural interactions between Bcl-2 and the transcription factor Twist1 in the relationship with metastasis and vascular mimicry in hepatocellular carcinoma., SNAIL and TWIST play a crucial role in epithelial to mesenchymal transition through regulation of E- and N-cadherin expression, exclusively in estrogen receptor-positive breast cancer patients., confirmed that down-regulation of TWIST expression decreased the motion, invasion, and migration ability of the Hep-2 cells, TWIST and SNAI1 expression in stromal cells is associated with clinical and histopathological characteristics that indicate progressive disease. Negative expression of these EMT-promoting transcription factors predicts a better outcome., Cancer-associated fibroblasts in gastric cancer frequently have altered Twist1 expression., expression was positively associated with lymph node metastasis, pathologic grade, and tumor stage [in oral squamous cell carcinoma], Twist1 and Twist2 modulate ATF4-dependent transcriptional activity in response to Parathyroid hormone., Twist1 directly activates Bmi1 expression and that these two molecules function together to mediate cancer stemness and epithelial-mesenchymal transition--{REVIEW}, these findings strongly implicate an essential role for TWIST1 in the initiation of human decidualization and uterine stromal cell apoptosis that occurs upstream of the induction of forkhead box protein O1 and v-ets-erythroblastosis virus E26 mRNA., Epithelial-mesenchymal transition and cancer stemness, the important role of hypoxia-mediated signalling in osteogenic differentiation in MSCs through direct regulation of RUNX2 by TWIST, SET8 and TWIST are functionally interdependent in promoting EMT and enhancing the invasive potential of breast cancer cells in vitro and in vivo, Twist is an upstream regulator of the E-cadherin-mediated terminal differentiation and fusion in a human trophoblastic cell line in vitro., down-regulation of the Twist gene suppressed the proliferation of MKN45 gastric cancer cells by negatively regulating the AP-1 activity, in both nontumorigenic and tumorigenic epithelial cancer cells, that Snail1 is uniquely required for EMT initiation, whereas Twist1 is required to maintain late EMT, Overexpression of MAML-1 and Twist1 were significantly associated with lymph node metastasis and the surgical staging of tumor, Overexpression of TWIST may correlate to tumor invasion in gastric adenocarcinoma., CCN5-induced inhibition of TWIST1 expression is mediated through the translational inhibition/modification of hypoxia-inducible factor-1alpha via impeding JNK signaling pathway., nucleostemin expression level correlated with TWIST expression level in esophageal cancer cell lines; identified that high nucleostemin proportion, TWIST intensity, and advanced pathological N stage were correlated with poor relapse-free survival, regulation of ER by Twist could be an underlying mechanism for the loss of ER activity observed in breast tumors, and may contribute to the generation of hormone-resistant, ER-negative breast cancer, Twist recruits DNA methyltransferase 3B (DNMT3B) and histone acetylase 1 (HDAC1) to the estrogen receptor promoter, which reduces the estrogen receptor expression in breast cancer., Twist transcriptionally regulates estrogen receptor alpha expression., ARTN and TWIST1 synergize to produce a worse outcome in ER-MC and combined inhibition of ARTN and phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) may therefore provide a novel therapeutic strategy in this subtype of mammary carcinoma., Human TWIST was found to be endogenously expressed in human fetal myoblasts, and its expression decreased during late stages of development., bladder cancer T24 cells may undergo the telomerase-to-ALT-like conversion and promote cancer progression, Twist1 expression, owing to the abnormal NPM-ALK/STAT3 signaling, contributes to its invasiveness in ALK-positive anaplastic large cell lymphoma., Twist is an upstream regulator of N-cadherin-mediated invasion of human trophoblastic cells., Increased expression of Twist was observed in 38.0% of primary NSCLC tumors. Overexpression of Twist in stage I NSCLC was associated with a worse overall survival and correlated with a shorter recurrence-free survival., Glioblastoma multiforme tissue grade IV specimens showed significant expression of Twist1 and Sox2, known mesenchymal and stemness related markers, respectively, indicating their association with glial tumor genesis and metastasis., Regulation of transcription factor Twist expression by the DNA architectural protein high mobility group A2 during epithelial-to-mesenchymal transition., *uggest that Twist1-mediated modulation of MDR1/P-gp expression plays an important role in sensitization of cervical cancer cells to cisplatin., our results establish a signaling axis through which the tumor microenvironment elicits Twist1 expression to promote cancer metastasis. We, miR-580 is able to target the promoter region of TWIST1 and to control TWIST1 expression., Results suggest that TWIST1 is an important driver of epithelial to mesenchymal transition in EGFR mutated cells and could be a potential marker in clinics to predict outcome in patients with EGFR mutated tumors., None of 20 ordinary glioblastomas showed TWIST1 expression in >10% neoplastic cells., These studies for the first time link Twist-1 to histone methylation of the Ink4A/Arf locus by controlling the expression of histone methyltransferases as well as the expression of other basic helix-loop-helix factors., TWIST may act upstream of E-cadherin, which can indirectly regulate the expression levels of beta-catenin in bladder cancer., TWIST-1 is constitutively degraded in EOC stem cells, and that the acquisition of TWIST-1 requires additional signals that will trigger the differentiation process, A potential role of TWIST as the promoter of tumor invasion and metastasis associated with down-regulation of E-cadherin., The results uncover an essential mechanism to explain how Twist1 induces the motile stem-like cancer cell phenotype beyond simply suppressing E-cadherin., our study indicates that nuclear twist plays an important role in ESCC lymphatic metastasis by increasing the expression of VEGF-C., TWIST expression was inversely correlated with ERalpha expression in both breast cancer cell lines and human breast ductal carcinomas., Increased Twist protein expression in advanced MF/SS lesions suggests that Twist expression may correlate with MF/SS stages., identify Twist1 as an inhibitor of Sox9 and further suggest that the balance between Twist1 and Sox9 may determine the earliest steps of chondrogenesis., Three novel TWIST1 mutations were detected, and p.P250R was the only mutation in FGFR3 associated with craniosynostosis., Twist was expressed significantly more and E-cadherin significantly less in gastrointestinal stromal tumors (GISTs) with metastasis, and expression of both was closely related to metastasis of GISTs., Twist may be associated with the epithelial-mesenchymal transition in gastric cancer and the tumorigenesis, invasion, and metastasis of gastric cancer., results suggest that Notch1/STAT3/Twist signaling axis is involved in progression of human gastric cancer and modulation of this cascade has potential for the targeted combination therapy, The suppression of TWIST1 in human lung cancer cells also induced cellular senescence, these results indicate that Twist1 induces the repression of claudin-4 expression during the epithelial-mesenchymal transition in esophageal carcinoma., Depletion of TWIST1 in MMSET-overexpressing cells blocked cell invasion and epithelial-mesenchymal transition, indicating that TWIST1 is a critical target of MMSET, responsible for the acquisition of an invasive phenotype., Dysregulated expression of TWIST1, TGF-beta1 and SMAD3 mRNA observed in osteoarthritis bone is reflected in the functionality of the osteoblast when these cells are cultured ex vivo., Our results suggest that Twist was expressed significantly more and E-cadherin significantly less in osteosarcoma with metastasis, findings indicate that CLU is an important mediator of TGF-beta-induced EMT, and suggest that CLU suppression may represent a promising therapeutic option for suppressing prostate cancer metastatic progression, we have demonstrated strong diffuse expression of SNAI2 and TWIST1 in the majority of anaplastic thyroid carcinomas but not in normal thyroids, thyroid adenomas or well-differentiated thyroid carcinomas, These results indicated that moscatilin inhibited MDA-MB-231 cell migration via Akt- and Twist-dependent pathways; this finding was consistent with moscatilin's antimetastatic activity in vivo., Letter/Case Report, TWIST1 mRNA expression is an independent prognostic factor for poor prognosis in LNN/ER-positive breast cancer., Ectopic expression of Snail and Twist contributed to lymph node and disseminated metastasis, respectively, by reducing E-cadherin expression, providing a novel role for Snail and Twist in the progression of colorectal cancer., The assessment of the Twist immunoreactivity and lymphovascular space involvement may distinguish high- from low-risk patients with locally invasive cervical carcinoma., study found that Twist1 acts to inactivate p53 in mesenchymal tumors; evidence provided that Twist1 binds p53 C terminus through the Twist box; this interaction hinders key posttranslational modifications of p53 and facilitates its MDM2-mediated degradation, PROX1 directly binds to proximal promoter of TWIST1 gene to repress its transcription, TWIST-1 and miR10a/b expression are dysregulated in MDS. Expression of miRs10a/b is controlled by TWIST-1., The overexpression of SLUG and TWIST with down-regulation of E-cadherin was characteristic findings in hemangiopericytomas and solitary fibrous tumors, but not in meningiomas., It might play an important role in the implantation of metastatic foci., Methylation levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 in urine specimens are promising diagnostic biomarkers for bladder cancer recurrence surveillance, ARTN promotion of the cancer stem cell-like cell phenotype was mediated by TWIST1 regulation of BCL-2 expression., This study study showed that HMGA2 was able to elicit EMT and regulate several genes which are closely related to the Wnt/beta-catenin pathway by directly binding to their promoter., high expression of TWIST1 and TWIST1 promoter methylation were significantly more frequent in borderline/malignant phyllodes tumors (PT) than in benign PT; results suggest a high expression of TWIST1 and related proteins plays a pivotal role in the malignant progression of PT, The expression of Twist1 has a close relationship with MMP-2 in endometrial endometrioid adenocarcinoma. Twist1 may be closely correlated with the tumor invasion, metastasis and prognosis., HBx activates the Twist promoter by activating STAT3 and promotes epithelium-mesenchymal transition occurrence in liver cells., Twist is essential for epithelial-mesenchymal transition, invasion, and metastasis of ovarian cancer., A previously unrecognized interplay between ERK1/2, TWIST1, and MMP-1 that is likely significant in the progression of melanoma toward metastasis., Twist1 promotes tumor invasion by down-regulating E-cadherin expression., TWIST interacts with beta-catenin signaling on osteosarcoma cell survival against cisplatin., our findings show that how Twist1 recruits stromal macrophages through CCL2 induction to promote angiogenesis and tumor progression., Hence, the dosage of TCF12-TWIST1 heterodimers is critical for normal coronal suture development, TWIST1 was repressed in patients with dilated cardiomyopathy compared to controls., Together these findings suggest that silencing of TWIST1 in oncogene driver-dependent NSCLCs represents a novel and promising therapeutic strategy., Data observed that depletion of beta-TRCP leads to an accumulation of Twist protein, which could enhance tumor cell motility and cancer metastasis., Twist1 and YB-1 promote bladder cancer progression, PLS2, Twist KIR3DL2 and NKp46 gene expression can model efficient molecular Sezary syndrome diagnosis., down-regulation of Twist expression by PGPW1 blocked epithelial-mesenchymal transition (EMT), characterized by a gain of epithelial cell markers, E-cadherin, and loss of the mesenchymal markers, vimentin and N-cadherin, at protein levels., this study highlights the determinant role of the interhelical loops in maintaining the TWIST1-DNA complex structures and provide a structural explanation for the loss of function associated with several TWIST1 mutations/insertions observed in Saethre-Chotzen syndrome patients, Studies indicate that mesenchymal stem cells (MSC)-derived hyaluronan (HA) was sufficient and necessary to induce lysyl oxidase (LOX) and Twist in the cancer cells., Twist1 gene may play a role as an oncogene during the tumorigenesis and development in bladder urothelial carcinoma., The expression of Twist is upregulated, whereas that of E-cadherin is downregulated in ovarian cancer. EMT, mediated by Twist, may be correlated with ovarian cancer metastasis., TGF-beta1 up-regulates STAT3 activation and HIF-1alpha stabilization, which leads to TWIST1 expression in a prostate cancer invasion cascade, TWIST1 promoter hypermethylation may be a useful molecular marker for predicting prognoses and contralateral cervical lymph node metastases in patients with tonsillar squamous cell carcinoma., combined methylation analysis of Twist homolog 1 and nidogen-2 genes may be a simple, noninvasive, sensitive, and specific method for detecting cancer cells in urine., Invasive neoplastic cells with a fibroblastlike morphology are traceable as TWIST1-positive elements in the peritumoral stroma of human colorectal cancer, and TWIST1 mRNA levels are increased in the blood of colorectal cancer patients., WAVE2 complex regulates breast epithelial morphology by a complex mechanism involving repression of Twist1 expression and Abl kinase activity, Lipocalin-2 negatively modulates the epithelial-to-mesenchymal transition in hepatocellular carcinoma through the epidermal growth factor (TGF-beta1)/Lcn2/Twist1 pathway., The proto-oncogene TWIST1 is regulated by microRNAs., Twist1 (and YB-1, androgen receptor) signaling promote docetaxel resistance in castration-resistant prostate cancer cells., Sox2 regulates the invasiveness of breast cancer cells via a mechanism that is dependent on Twist1 and the transcriptional status of Sox2, Loss of TWIST1 gene is associated with a saethre-chotzen-like phenotype, severe intellectual disability, and autism, Increased expression of both TWIST1 was associated with decrease renal function in IgA nephropathy., Our study suggests that Twist, as an important potential downstream effector, plays a key role in TFF3-modulated metastasis in gastric cancer and can be a promising therapeutic target against intestinal-type gastric cancer., Twist1 as a common downstream target of multiple signalling cascades plays a crucial role in mediating drug resistance to BRAF- and MEK-targeted molecular inhibitors., Twist is responsible for the stemness of cancer cells and the generation of drug resistance. [Review], MEK1/2 inhibition caused approximately twofold decrease in Twist serine phosphorylation, Ex vivo expansion protocols involving hypoxic preconditioning inhibit cell senescence in endothelial progenitor cells by hypoxia-induced hypoxia-inducible factor-1alpha-TWIST-p21 axis., identify the transcription factor Twist1 as a component of a STAT3-induced feedback loop that controls IL-6 signals by directly repressing Il6ra., Data suggest that the Twist box domain is required for Twist1 transcriptional programs and prostate cancer metastasis., Aberrant expression of p53, Twist, and E-cadherin is found in patients with oral squamous cell carcinoma., Data indicate that E-cadherin was elevated , and the expression levels of N-cadherin, Vimentin and Twist1 were all decreased in oleanolic acid (OA)-treated cells., Up-regulation of TWIST1 is associated with oral sub-mucous fibrosis., Overexpression of TWIST1 is associated with invasive endometrial cancer., Twist1-Tie2-Angs signaling is important for controlling vascular permeability, Twist1 is an essential transcription factor for the maintenance of mesenchymal characters and tumor initiating properties of synovial sarcoma, Interleukin-6-induced Twist and N-cadherin have roles in melanoma cell metastasis, High TWIST1 expression is associated with breast cancer., At the molecular level, we demonstrated that HDAC5 promoted mRNA expression of twist 1, which has been reported as an oncogene, These findings first determine that Twist contributes to invasion and metastasis by inducing wide-ranging transcriptional and functional changes of long non-coding RNAs and signal pathways in our study., determined whether activation and expression levels of EGFR1, ERK, AKT, STAT3, and TWIST1 are dependent on activating mutations of EGFR1 in NSCLC, this study demonstrates that miR-181a-Twist1 pathway may play an important role in the development of cisplatin-chemoresistance, with EMT and an increase the metastatic potential of tongue squamous cell carcinoma cells., result was corroborated by the clinical observation that Twist1 up-regulation predicted the occurrence of lymph node and lung metastases as well as poor patient survival, Data show that modin directly inhibited TWIST1 expression, upregulated E-cadherin, and inhibited TWIST1-induced epithelial-mesenchymal transition (EMT) by inhibiting the beta-catenin and Akt pathways., Twist 1 could bind to the promoter region of FoxM1., KLF17 may have an oncogenic role during EEC progression via initiating EMT through the regulation of TWIST1, Overexpression of HIF-2alpha, TWIST, and CXCR4 is associated with lymph node metastasis in papillary thyroid carcinoma., twist and zeb1 are involved in placental maturation, whereas claudin 4 appears to be connected with placental diseases such as diabetes, toxaemia or molar disease., High TWIST1 and SNAI1 mRNA expression was associated with poor overall survival in human colorectal cancer., upregulation and senescence bypass during progression and metastasis of cervical cancer, Id4 has a role in suppressing MMP2-mediated invasion of glioblastoma-derived cells by direct inactivation of Twist1 function, Studied the expression of E-cadherin, N-cadherin, TGF-beta1 and Twist protein and investigate its significance in the occurrence and development of prostate cancer., TWIST1 mediated EGF-induced N-cadherin expression, leading to prostate cancer invasion., The combination of gamma-catenin and Twist positive staining is associated with poor prognosis in malignant pleural mesothelioma., MiR-33a promotes osteosarcoma cell resistance to cisplatin by down-regulating TWIST., Kindlin-3 influences breast cancer progression by influencing the crosstalk between beta1 integrins and Twist to increase VEGF production, which enhances breast cancer cell invasion and tumor angiogenesis and metastasis, Insulin-like growth factor-I induces CLU expression through Twist1 to promote prostate cancer growth., depletion of Sox5 in breast cancer cells caused a dramatic decrease in Twist1 and chromosome immunoprecipitation assay showed that Sox5 can bind directly to the Twist1 promoter, suggesting that Sox5 transactivates Twist1 expression, SRC1 and Twist1 expression are associated with poor survival in breast cancer., The overexpression of Twist1 protein enhanced the resistance to apoptosis, with apoptotic proteins changing to resist cell death, and inhibited Ca2+ release induced by taxol, High Twist1 expression is associated with mesenchymal tumors., We showed that Twist1 affects thyroid cancer cell survival and motility., TWIST1 demonstrated marked down-regulation in basal cell carcinoma compared to normal skin tissue., miR-300 inhibits epithelial to mesenchymal transition and metastasis by targeting Twist in human epithelial cancer., High Twist1 expression is associated with squamous cell carcinoma., TIMP-1's function as an endogenous inhibitor of MMP or as a "cytokine-like" signaling molecule may be a critical determinant for tumor cell behavior., It is a epithelial-mesenchymal transition promoting transcription factor and its expressionis associated with aggressiveness and poor prognosis of intraductal papillary mucinous neoplasm of pancreas., Twist1 is a key downstream effector of p62 in regulation of cell proliferation and migration, TWIST1 inhibited TRIM29 promoter activity., Twist plays important roles in tumor growth, progression, and survival in patients with renal cell carcinoma patients, FOXD3 and TWIST1 define distinct subgroups of cells within a heterogeneous tumor., Twist1-Jagged1/KLF4 axis is essential both for transdifferentiation of tumour cells into endothelial cells and for chemoresistance acquisition., A single cell methods shows that high expression of ALDH1 along with nuclear TWIST in circulating tumour cells are frequently detected in patients with metastatic breast cancer., these results demonstrated for the first time that SIRT6 suppressed non-small cell lung cancer cells proliferation via down-regulation of Twist1 expression, Elevated serum levels of Twist were associated with higher breast cancer staging and lymph node metastasis., CCL2 greatly enhanced IL-6-induced EMT events mainly by upregulating the expression of Twist. Genetic or pharmacological inhibition of STAT3 disrupted STAT3-centered loop and markedly suppressed Twist expression as well as IL-6/CCL2-mediated EMT induction, loss of BRMS1 promotes malignant phenotypes that are dependent on NF-kappaB-dependent regulation of Twist1, Twist-related protein 1 enhances oral tongue squamous cell carcinoma cell invasion through beta-catenin signaling., LMP2A may suppress the HER2 expression through the TWIST/YB-1 axis in Epstein-Barr virus-associated gastric carcinoma, TWIST1 is a novel regulator of pathologic ocular angiogenesis and may represent a new molecular target for developing potential therapeutic treatments to suppress pathological neovascularization in vascular eye diseases., miR-520d-5p targeting of TWIST1 resulted in reduced expression of the prominent metastamiR miR-10b, A systematic review and meta-analysis were performed to determine whether the expression of Twist-1 was associated with the prognosis of carcinoma patients., DEC2 regulates cellular function by modulating the expression of Twist1, MEOX2 participates in chemoresistance irrespective of high CNV, but it is significantly dependent upon H3K27me3 enrichment probably associated with aggressiveness and chemotherapy failure in NSCLC patients, TWIST1 promoter hypermethylation is associated with Colorectal Cancer., Author demonstrate that both N-Myc and c-Myc proteins can bind and activate the TWIST1 promoter and propose TWIST1 as a direct MYC transcriptional target., miR-23a promotes cisplatin chemoresistance and protects cisplatin-induced apoptosis in TSCC cells through inducing Twist expression by a JNK-dependent mechanism, This review describes that d-acetylated Twist recruits BRD4 during progression of basal-like breast cancer., Data suggest that TWIST1 and to a lesser degree TWIST2 expressed within the tumor stroma could contribute to the epithelial-mesenchymal transition (EMT)-like tumor budding phenotype in colorectal cancers., Twist1 and FGFR2 are highly associated with differentiation of gastric adenocarcinoma; Twist1 can facilitate invasion and EMT in gastric adenocarcinoma via promotion of FGFR2 expression., we revealed that SOX5 is linked to epithelial-mesenchymal transition in hepatocellular carcinoma by regulation of Twist1, Twist1 is required for skin tumor initiation and progression in a gene-dosage-dependent manner., Twist1, together with snail, links the Hedgehog signaling pathway to the tumor-initiating cell-like properties of chemoresistant cells., This study reports the first evidence of miR-10b over-expression in NPC patients. Furthermore, our findings can support hsa-miR-10b gene regulation through LMP1/Twist1 in NPC malignancy, Twist1 expression positively correlates with integrin alpha5 expression in human colorectal cancers.Twist1 and AP-1 cooperatively upregulate integrin alpha5 expression to induce invasion and the epithelial-mesenchymal transition., TWIST1 TQS phosphorylation state determines the intensity of TWIST1-induced pro-metastatic ability in prostate cancer cells, which may be partly explained mechanistically by TWIST1 dimeric partner choice., STAT3 may bind the Twist promoter, mediate its transcriptional activity, and then promote the EMT process in SMMC7721 cells., TWIST1 and TWIST2 are expressed in skeletal muscle and remained unaltered in metabolic diseases., The data suggest that TWIST over-expression has a correlation with lymphatic and distant metastasis in nasopharyngeal carcinoma, Twist and Foxc2 stromal expression in PTs was significantly correlated with tumour grade and worse histological features., To drive STAT3 activation and EMT through STAT3-mediated TWIST1 induction., Up-regulated Sox12 induced by FoxQ1 promotes hepatocellular carcinoma invasion and metastasis by transactivating Twist1 and FGFBP1 expression., Breast cancer patient miR-424 levels positively associate with TWIST1/2., Twist gene promotes the formation and migration of mammospheres in breast cancer cells., Leading to significant knockdown of TWIST1., PDGF-D expression is associated with miR-106a and Twist1 in HCC patients, Twist gene silencing significantly induced apoptosis, cell cycle arrest at G0/G1 phase, proliferation inhibition, and reduced the ability of migration and invasion in human gastric cancer SGC7901 cells., High TWIST1 expression is associated with gastric cancer., the EMT phenotype was detected in cisplatin-resistant ovarian cancer tissues and cell lines, and correlated with decreased miR-186 expression, increased Twist1 expression, chemoresistance and poor prognosis in epithelial ovarian cancer, targeting miRNA by cordycepin indicates a new mechanism of cordycepin-induced suppression of tumor metastasis; miR-33b/HMGA2/Twist1/ZEB1 axis plays critical roles in regulating melanoma dissemination, These results indicate that tamoxifen can significantly accelerate Twist1 degradation to suppress cancer cell invasion and metastasis, regardless of the estrogen receptor status., Reveal a TWIST1-G3BP2 mechanotransduction pathway that responds to biomechanical signals from the tumour microenvironment to drive EMT, invasion and metastasis., MACC1 promotes vasculogenic mimicry in gastric cancer by regulating the HGF/c-Met-TWIST1/2 signaling pathway., Increased expression of TWIST1 is associated with metastatic capability of immunoedited tumor cells., The mechanism through which miR-33b inhibits the stemness, migration and invasion of breast cancer cells is by targeting HMGA2, SALL4 and Twist1., Fetuses with ventricular septal defects have decreased TWIST1 mRNA and protein expression levels in the myocardial tissue., TWIST1 has a potential causative effect on the development of ventricular septal defect., Twist promotes pancreatic cancer cell invasion and cisplatin chemoresistance through inducing GDF15 expression via a p38 MAPKdependent mechanism., After chronic NOD2 stimulation, Twist1 and Twist2 coordinate the regulation of both transcriptional activators and repressors, thereby mediating optimal cytokine down-regulation., Thymoquinone treatment inhibits TWIST1 promoter activity and decreases its expression, leading to the inhibition of cancer cell migration, invasion and metastasis., TWIST-1 has a role in promoting cell growth, drug resistance and progenitor clonogenic capacities in myeloid leukemia, Akirin-2 downregulation increases chemosensitivity in human glioblastomas more efficiently than Twist-1, HGF and TGFbeta1 of bone-metastasis microenvironment acted co-ordinately, influencing non redundant pathways regulated by Twist program or Snail-transcription factor, with reversible MET switch, Twist contributes to peritoneal mesothelial cell fibrosis during peritoneal dialysis treatment, mainly through regulation of YB-1., EGF receptor promotes prostate cancer bone metastasis by downregulating miR-1 and activating TWIST1.miR-1 directly targets the 3'UTR of TWIST1 and regulates TWIST1 mRNA stability., when siRNA against TWIST1 was delivered via mesoporous silica nanoparticle, there was tumor reduction in an in-vivo model., SLUG and TWIST expression in IDC was significant higher in stromal cells., our work revealed that MTDH promotes CSC accumulation and breast tumorigenicity by regulating TWIST1, deepening the understanding of MTDH function in cancer., Twist is upregulated in glioblastomas, Data showed a lack of association between circulating tumor cells (CTCs) and expression of epithelial to mesenchymal transition (EMT) factors, TWIST1 and SLUG, in breast tumor suggesting that expression of EMT protein is not surrogate marker of CTCs., Activation of the miR-373-TXNIP-HIF1alpha-TWIST signaling axis is correlated with a worse outcome in patients with breast cancer., We found that TWIST1, SNAIL and SLUG are overexpressed in endometriosis, Hypoxia upregulated the expression of Twist1, Bmi1, epithelialmesenchymal transition markers, stem cell markers and vasculogenic mimicry associated markers in hepatocellular carcinoma cells., co-expression of TWIST1 and ZEB2 in OSCC was significantly associated with poorer overall survival, particularly in patients without detectable lymph node metastasis., The results suggested a novel function for Twist in the promotion of EMT via TGF-b/Smad3 signaling pathway. Thus, Twist constitutes a potential therapeutic target in human cervical cancer., Over-expressed TWIST associates with markers of epithelial mesenchymal transition and predicts poor prognosis in breast cancers via ERK and AKT activation., Twist1 mediates TGF-beta-induced expression of both full-length and splice-variant AR expression. Twist1/AR signaling was augmented in castration resistant as well as mesenchymal-phenotype prostate cancer., The downregulation of ZEB1, Twist1, and Snail1 reduces the invasive properties of uveal melanoma cells, and the elevated mRNA levels of ZEB1 and Twist1 are associated with a more aggressive clinical phenotype in uveal melanoma samples., provide evidence that Bcl-2 enhances the role of Twist-1 in vasculogenic mimicry and provide evidence that Bcl-2 enhances the role of Twist-1 in vasculogenic mimicry and epithelial-mesenchymal transition through miRNAs through miRNAs, Twist promotes reprogramming of glucose metabolism in MCF10A-Twist cells and Twist-positive breast cancer cells via activation of the beta1-integrin/FAK/PI3K/AKT/mTOR pathway and inhibition of the p53 pathway., Twist1 is correlated with p-4E-BP1 in predicting the prognostic outcome of NSCLC., A detailed analysis of the TWIST1 promoter showed that SP1 was required for the basal transcription of TWIST1 and that BHLHE40/41 suppressed SP1 binding to the consensus SP1 binding site of the TWIST1 promoter., Twist immunohistochemical analysis can be used as an adjuvant to H&E histopathological findings for proper categorization and grading of ameloblastoma especially in the clinically aggressive tumours., highly expressed in stromal villi cells of molar disease, Our results identified TWIST1 as a transcription factor that regulates C3 expression during pathologic and physiologic epithelial-mesenchymal transition, Study found that upregulated Twist1 increased Bmi1 expression in acute myeloid leukemia (AML) and endued leukemic cells a higher proliferative potential and increased resistance to apoptosis. In primary AML samples, there was strong positive correlation between the expression levels of Twist1 and Bmi1., findings not only pinpoint that Twist1 mediates the modulatory function of PAQR3 on EMT and metastasis but also suggest that targeting Twist1 is a promising strategy to control metastasis of tumors with downregulation of PAQR3, TWIST1 gene mutation maybe an underlying factor in the development of craniosynostosis., expression of ZEB1 and TWIST1, which are master regulators of tumor metastasis. Our study first indicates that miR-1271 functions as a suppressor in regulating of pancreatic cancer EMT by targeting ZEB1 and TWIST1.,
OMIM_DISEASE Saethre-Chotzen syndrome, Saethre-Chotzen syndrome with eyelid anomalies, Craniosynostosis, type 1, Robinow-Sorauf syndrome,
SP_COMMENT disease:Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly., disease:Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape., disease:Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome., function:Probable transcription factor, which seems to be involved in the negative regulation of cellular determination and in the differentiation of several lineages including myogenesis, osteogenesis, and neurogenesis. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins (By similarity). Also represses expression of proinflammatory cytokines such as TNFA and IL1B., similarity:Contains 1 basic helix-loop-helix (bHLH) domain., subunit:Efficient DNA binding requires dimerization with another bHLH protein. Homodimer., tissue specificity:Subset of mesodermal cells.,