Database for Annotation, Visualization and Integrated Discovery 2.1
National Institute of Allergy and Infectious Disease
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CREB binding protein(CREBBP) CREB binding protein(CREBBP) Related Genes Homo sapiens
CHROMOSOME 16,
CYTOBAND 16p13.3,
ENSEMBL_GENE_ID ENSG00000005339,
ENTREZ_GENE_ID 1387,
GENERIF_SUMMARY substrate specificity; structure activity relationship, The PHD type zinc finger is an integral part of the CBP acetyltransferase domain, Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation., IL-6-inducible expression of the hAGT promoter is mediated by physical association of the COOH terminus of STAT3 with p300/CBP, the recruitment of which targets histone acetylation and results in chromatin remodeling., overexpression of CBP is detected from the very early stages of laryngeal carcinogenesis, suggesting that CBP may play a role in malignant transformation of precancerous laryngeal lesions, role in acetylation of beta-catenin, Regulatory motifs for CREB-binding protein and Nfe2l2 transcription factors in the upstream enhancer of the mitochondrial uncoupling protein 1 gene., A missense mutation has been identified in codon 1175 of CREBBP in a mild case of Rubinstein-Taybi syndrome., HIV Tat is a general inhibitor of histone acetylation by cellular HATs and for the CREB-binding protein (CBP), it induces a substrate selectivity, CBP enhances tumor necrosis alpha-induced cell death in rheumatoid synoviocytes, role in transcriptional control of the inflammatory response, is co-expressed with CREB and CBP in extravillous cytotrophoblasts, revealing the in vivo relevance of this transactivation pathway, results suggest that all-trans-retinoic acid and retinoic acid receptors regulate growth arrest of human mammary epithelial cells and modulate CBP/p300 protein expression, arginine methylation represents an important mechanism for modulating CBP co-activator transcriptional activity, Oct-1 potentiates CREB-dependent cyclin D1 transcriptional activity by a phospho-CREB and CREB binding protein-independent mechanism, CBP has a role in regulating 5-aminolevulinate synthase gene expression via the AP-1 complex in human tumor cells, structurally distinct modes of recognition of the KIX domain of this protein by Jun and CREB, interaction of CREB-binding protein with EWS selectively activates hepatocyte nuclear factor 4-mediated transcription, The CBP/p300 acetylase and the CARM1 methyltransferase can positively regulate the expression of estrogen-responsive genes, there is a crosstalk between lysine acetylation and arginine methylation on chromatin, recruitment to pp90RSK regulates cAMP response element-binding protein activity which is negatively affected by ERK phosphorylation, The KID-interacting domain of human coactivator CREB-binding protein (CBP) is the CBP domain that is targeted by HIV-1 Tat during HIV-1 propagation., PHD finger mutations cause a loss of CBP acetyltransferase activity, in human mammary epithelial cells, CBP/p300 were both modulated by an all-trans-retinoic acid (ATRA) signaling pathway and were required for a normal response to ATRA, deletion of the CBP bromo- and C/H3 domains eliminates stimulation of nucleosomal histone deacetylase activity in vitro and transcriptional coactivation by EBV Zta in transfected cells, Loss of CREBBP was not statistically significant selection in cancer cells stratified by various criteria for the concordant loss of EP300 and CREBBP., CBP and p300 function as co-activators of Sox9 for cartilage tissue-specific gene expression and chondrocyte differentiation., p34SEI-1 strongly suppressed CREB-mediated transcription, and this suppression was overcome by excess amount of CBP, Estrogen receptor-alpha represses human GnRH receptor gene transcription via an indirect mechanism involving Creb-binding protein., CBP-induced acetylation of AFX is a novel modification mechanism by which AFX keeps the transcriptional activity mitigating in the nucleus, Loss of heterozygosity and internal tandem duplication mutations of the CBP gene are frequent events in human esophageal squamous cell carcinoma, The site of the KID-interacting (KIX) domain of CBP that recognizes c-Jun and mixed lineage leukemia MLL proteins is identified as the same site that binds HIV-1 Tat protein., the conserved bromo-domain of the transcriptional coactivator CBP (CREB binding protein) binds specifically to p53 at the C-terminal acetylated lysine 382, Novel heterozygous deletion in CREBBP in Rubinstein-Taybi syndrome patient results in the loss of exon 30., activity of ESE-1 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP., activation of CREB-binding protein and inhibition of MAPK has a role in cAMP-dependent protein kinase type I regulation of ethanol-induced cAMP response element-mediated gene expression, CBP acetylation controls HNF6 protein stability, effect of CREB-binding protein on inhibition of Smad-mediated transcriptional activation by K-bZIP was examined, E2A-PBX1 interacts directly with the KIX domain of CBP/p300 in the induction of proliferation in primary hematopoietic cells, important functions in coordinated cell cycle progression, Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment, Both TAZ domains of CREB-binding proteins CBP and p300 in the presence of stoichiometric concentrations of zinc adopt a well-defined structure in solution in the absence of binding partners., Mutations and deletions of the CBP gene is associated with lung cancer, glutamine rich and bZIP domains stabilize CREB binding to chromatin, In 92 patients, we were able to identify a total of 36 mutations in CBP. By using multiple ligation-dependent probe amplification, we found not only several deletions but also the first reported intragenic duplication in a patient with RSTS., HIF-1alpha, STAT3, CBP/p300 and Ref-1/APE regulate Src-dependent hypoxia-induced expression of VEGF in pancreatic and prostate carcinomas, Smad-binding peptide aptamers can be developed to selectively inhibit TGF-beta-induced gene expression., In conclusion, this research supports FSCN1 as a novel marker of NT2 neuronal differentiation and the possible role of CBP in its regulation., The interaction of fibroblast growth factor receptor-1, The identification of CBP inactivating mutations in conjunction with disruption of the second CBP allele strongly suggests that CBP is a tumour suppressor gene that contributes to ovarian tumorigenesis in humans., We aimed to determine the rate of point mutations and other small molecular lesions in true Rubinstein-Taybi syndrome and possible mild variants, by using genomic DNA sequencing of the CREBBP gene., CBP and PCAF coactivators mediate ERK1 gene expression at both the transcriptional and post-transcriptional level, the histone chaperone SET regulates CBP-mediated transcription, The interaction between CtBP and CBP is functionally important and leads to impairment of histone H3 acetylation by CBP at specific lysine residues (Lys9, Lys14, and Lys18) in a dose-dependent and NADH-dependent manner., GCMa acetylation is mediated by CBP, which stimulates GCMa transcriptional activity through cyclic AMP/protein kinase A signaling, CREB elements on the COX-2 promoter were involved in the gene transcription event., Suppression of CBP in human mammary epithelial cells results in loss of reconstituted extracellular matrix-mediated growth control and apoptosis and loss of laminin-5 alpha 3-chain expression., both p300 and CBP increase ATF4 transcriptional activity, arginine methylation of CBP is required for IFN-gamma induction of MHC-II. A kinetic analysis shows that CIITA, CARM1, and H3-R17 methylation all precede CBP loading on the MHC-II promoter during IFN-gamma treatment., Recruitment of Daxx following SUMO modification represents a previously undescribed mechanism in modulating CREB-binding protein (CBP) transcriptional potential., ATM is required for CREB phosphorylation in UV irradiation-damaged cells., study proposes a new mechanism by which estrogen receptors and retinoic acid receptors regulate BRCA1-mediated DNA repair by means of CBP, analysis of a novel, noncanonical mechanism of modulation of beta-catenin signaling through direct phosphorylation of beta-catenin by PKA, promoting its interaction with CREB-binding protein, Expression of a dominant negative CREB (S133A) blocked PDB/TG-dependent induction of both COX-2 and mPGES-1 promoters. Decreased CREB expression by siRNA blocked PDB/TG-dependent expression of COX-2 and mPGES-1 mRNA., binding of p53 serves to modify simian virus 40 large T antigen (SV40 LT) by targeting CREB binding protein and p300 binding to direct the acetylation of SV40 LT, MOZ-TIF2 associates with the RARbeta2 promoter in vivo, resulting in altered recruitment of CBP/p300, aberrant histone modification, and down-regulation of the RARbeta2 gene, Stat3-mediated p100 processing to p52 requires activation of Stat3 by the acetyltransferase activity of cAMP-response element-binding protein (CREB)-binding protein (CBP)/p300, CREB phosphorylation and CRE-regulated gene expression are activated by kinases., Acid-induced NOX5-S expression and H(2)O(2) production were significantly inhibited by removal of extracellular Ca(2+) and by knockdown of CREB using CREB small interfering RNA in a tumor cell line., CREB activation is involved in up-regulation of Fc gammaRIIA expression in myeloid lineages., PEA3 and c-Jun stimulate synergistically the HER2/neu gene transcription with p300, histone deacetylase inhibitors not only inhibit deacetylase activity but also stimulate active NF-kappaB transcription and cell survival through signaling pathways involving Akt and increased p300 acetyltransferase activity, Our results provide evidence for novel functional interactions between the chromatin modification enzyme CBP and nuclear transport proteins in mammalian cells., A high detection rate of CREBBP mutations in Rubinstein-Taybi Syndrome patients is confirmed by this Italian study which also attests one of the highest microdeletion rate (16%) documented so far., first report of a direct interaction between N-CoR and CBP, and suggests that the role of N-CoR in mediating transcriptional events may be more complex than previously anticipated, hypothesized that HSV-1 ICP0 recruits activated IRF-3 & CBP/p300 to nuclear structures, away from host chromatin; this leads to inactivation & accelerated degradation of IRF-3, resulting in reduced transcription of IFN-beta & inhibition of host response, Large deletions including several exons are a relatively frequent cause of RTS; MP/LC is an effective method for detecting these deletions., Role for the CREB binding protein includes the modulation of chromatin structure and function during DNA metabolic events as well as for transcription., analysis of MYST3-CBP fusion transcripts in t(8;16)(p11;p13) acute myeloid leukemias, Coexpression of Etsl and CBP induced a synergistic activation of the parathyroid hormone-related protein P3 promoter only in the tumorigenic cell line., CBP/p300 has an evolutionarily conserved role as a buffer regulating TCF-beta-cat/Arm binding., Our results suggest that phosphorylation of CBP by IKKalpha regulates the CBP-mediated crosstalk between NF-kappaB and p53 and thus may be a critical factor in the promotion of cell proliferation and tumor growth., mice expressing a dominant-negative CREB transgene (M-ACREB mice) exhibited a dystrophic phenotype along with reduced myocyte enhancer 2 activity., P13-K/Akt-dependent activation of CREB protein in Jurkat T leukemia cells treated with TRAIL is reported., CBP and TRAP220 was diminished by the overexpression of a MED25 NR box deletion mutant, and by treatment with MED25 siRNA, finding implicates CBP as one of the causative genes for the trisomy 16p13 syndrome, and indicates this is a contiguous gene syndrome, results indicate that the genetic polymorphism of the CBP, which is one of the essential factors exerting the biological effects of glucocorticoids, may affect susceptibility to steroid-induced osteonecrosis in patients after renal transplantation, These results provide evidence for a model in which Tax and pCREB bind distinct surfaces of KIX for effective CBP/p300 recruitment to the HTLV-1 promoter., CREB binding to the HIF-1 responsive elements in PAI-1 promoter mediates the glucagon effect in the liver, Cyclosporine impairs CREB DNA binding activity., Therefore, these data support the hypothesis that CREB plays a critical role in the regulation of Bcl-2 expression in TCS-induced Hela cell death., High frequency of mosaic CREBBP deletions is associated with Rubinstein-Taybi syndrome, KLF4 might function as an activator or repressor of transcription depending on whether it interacts with co-activators such as p300 and CREB-binding protein or co-repressors such as HDAC3., Data demonstrates that CBP dosage is a critical regulator of gene-specific signal integration between the ER- and NFkappaB-signaling pathways., CREB binding proten affects Proteinase-activated receptor-2 and cyclooxygenase 2 expression., These data reveal that phosphorylation of CREB is not sufficient for CBP/p300 recruitment and transcriptional activation., the Hes6-CBP complex in PML-NB may influence the proliferation of cells via p53-dependent and -independent pathways., This study provides the first insight into the mechanisms that control basal expression of human SNF2L gene., The study establishes a molecular link between the role of serine 339 in IRF-3 homodimerization, CBP association, and its destabilization., CBP is a novel binding partner of XAF1, and the interaction between XAF1 and CBP and their functional consequence were mediated by adaptor-related protein complex 1., Three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) bound to peptides derived from histone acetylation sites., Phosphorylation deficient c-jun was less able to recruit PCAF to AP-1 sites., These results reveal that HDAg interacts with cellular transcription factor YY1 and its associated acetyltransferases CBP and p300 in a large nuclear complex, which in turn modulates the replication of HDV RNA., Results show that nuclear GAPDH is acetylated at Lys 160 by the acetyltransferase p300/CREB binding protein (CBP) through direct protein interaction, which in turn stimulates the acetylation and catalytic activity of p300/CBP., MUC5AC gene expression are mediated by a complex regulatory cascade controlling interactions between CREB and c-Ets1 that bind to a promoter element in the MUC5AC gene enhancing MUC5AC gene transcription., HTLV-1 Tax interferes with IFN-alpha-induced JAK-STAT pathway by competition with STAT2 for CBP/p300 binding., CBP duplications could result in the trisomy 16p13 phenotype., in patients with femoral head osteonecrosis, The synergistic index between the ABCB1 and CBP genes was >1.00 (1.99), revealing the presence of an interaction, PML (acute promyelocytic leukemia) nuclear bodies are nuclear sites involved in the ubiquitin-dependent degradation of CBP., Brucella controls host defenses by impairing macrophage responses to IFNgamma induced STAT1-CBP/P300 association., Results demonstrated that aberrant interaction of PHOX2B mutants with CBP and/or an interfering effect of certain PHOX2B mutants may be the critical mechanism to impair synergistic activation, thereby contributing to the phenotypes of CCHS., demonstrate that the histone acetyl transferase CBP (also known as Nejire) in flies and CBP and p300 (Ep300) in humans acetylate H3K56, The presence of an identified CREBBP gene abnormality was possibly related to the motor difficulties through impaired motor skills learning., Data support a model in which the NHD region of Pygopus is required to augment transcriptional activation by a mechanism that includes both transcriptional activation and histone acetylation resulting from the recruitment of the CBP., CBP-mediated post-translational N-glycosylation of BRCA2., Akt, activated downstream from ERK 1/2, was required for TGF-beta1-induced ZEBRA expression and enabled Smad3, a mediator of TGF-beta1 signaling, to be acetylated by direct interaction with the co-activator CREB-binding protein, Data show that cytoplasmic p300/CBP E4 activities ubiquitinate and destabilize p53, while physically separate nuclear p300/CBP activities, such as p53 acetylation, activate p53., Duplications of the critical Rubinstein-Taybi deletion region on chromosome 16p13.3 cause a novel recognisable syndrome., Here we present an 8-month-old boy with varicella meningoencephalitis, RTS, and a de novo deletion of the CREBBP gene of two base pairs at position 201-202 in exon 2, c. 201 202delT., This study demonstrated an association between two polymorphisms in the CBP gene and cognitive function in an elderly population., The aim of this genome-wide assay was to identify rearrangements that might underlie Rubinstein-Taybi syndrome-like phenotypes in patients who were negative at Fluorescence in situ hybridization analyses of the CREBBP and EP300 regions., Results describe the role of CREBBP re-arrangements during neuronal differentiation., DNA cytosine methylation in the bovine leukemia virus promoter has a role in direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcription factor binding, CCAAT/Enhancer-binding protein beta DNA binding is auto-inhibited by multiple elements that also mediate association with p300/CREB-binding protein, CREBBP variable expression (family 1) and somatic mosaicism (family 2) contribute to the phenotypic variability of Rubinstein-Taybi syndrome., Data report the highest detection rate of CREBBP mutations (76.9%) in Rubinstein-Taybi syndrome patients to date, of which ten are predicted to be pathogenic and three critical for histone acetyltransferase activity., Exon coverage microarray platform detected intragenic deletions, TNFalpha and IFNgamma synergistically enhance transcriptional activation of CXCL10 in human airway smooth muscle cells via STAT-1, NF-kappaB, and the transcriptional coactivator CREB-binding protein., p300 and CBP are implicated in regulation of ER-beta activity and cellular migration in prostate cancer., Data show that the binding rate between domains of CREB and CREB binding protein was initially accelerated and subsequently dramatically decreased as the strength of the nonnative hydrophobic interactions increased., BCL11B might play a role in anti-apoptosis in T-ALL cells through up-regulation of its downstream genes BCL2L1 and CREBBP., investigation of role of CBP and gamma catenin in regulation of transcription of survivin, Downregulation of CREBBP, seen in the intestine of celiac disease children, was normalized after gluten-free diet treatment., results identify CREBBP/EP300 mutations as a major pathogenetic mechanism shared by common forms of B-cell non-Hodgkin's lymphoma, with direct implications for the use of drugs targeting acetylation/deacetylation mechanisms, analysis of a cohort of 71 diagnosis-relapse cases and 270 acute leukaemia cases that did not relapse found 18.3% of relapse cases had sequence or deletion mutations of CREBBP, in estrogen-regulated gene programming, the acetyltransferase CREB-binding protein (CBP) is specifically and exclusively methylated by the coactivator-associated arginine methyltransferase (CARM1) in vivo, CDKN2A, GATA3, CREBBP, ITGA2, NBL1 and TGM4 were down-regulated in the prostate carcinoma glands compared to the corresponding normal glands, The results of this study suggested that rs3025684 polymorphism may be a possible risk factor for developing addiction., CBP/p300 and SIRT1 were recruited to histone gene promoters in an NPAT-dependent manner, knockdown of which affected histone acetylation on histone gene promoters and histone gene transcription., we identified different mutations at the CREBBP locus in nine patients with Rubinstein-Taybi syndrome that illustrate different grades of the clinical severity in the spectrum of the syndrome, CBP is required for regulation of BIRC3, and this may be due to its ability to acetylate histones around the estrogen response element and promote estrogen receptor access to the gene, thereby leading to potentiation of BIRC3 gene expression., The nuclear receptor co-activator binding domain (NCBD) of CREBBP selectively recruits transcription co-activators during formation of the transcription preinitiation complex. Three of four ligand-bound states are accessible to the ligand-free NCBDs., Selective roles for cAMP response element-binding protein binding protein and p300 protein as coregulators for androgen-regulated gene expression in advanced prostate cancer cells., CREBBP and EP300 mutations are not major pathogenetic mechanisms of myeloproliferative neoplasms, mutant p53 elevates expression of genes capable of enhancing cell proliferation, motility, and tumorigenicity by inducing acetylation of histones via recruitment of CBP and STAT2 on the promoters causing CBP-mediated histone acetylation, The rsults of this study suggested the lack of influence of SNPs under investigation in the present study on the susceptibility to schizophrenia and on the response to antipsychotics., our findings show that the E2A proteins are acetylated at specific residues as a consequence of lysine acetyltranferases(CBP, p300 and PCAF ) recruitment, TGF-beta1/beta-catenin/CBP-mediated pro-EMT signaling pathway., analysis of angiotensin II-dependent transcriptional activation of human steroidogenic acute regulatory protein gene by a 25-kDa cAMP-responsive element modulator protein isoform and Yin Yang 1, SRCAP is an SNF2-related chromatin-remodeling factor that serves as a coactivator for CREB-binding protein (CREBBP, better known as CBP, the major cause of Rubinstein-Taybi syndrome [RTS])., Rigorous genetic analysis of the CREBBP and EP300 genes using DNA sequencing and multiple ligation-dependent probe amplification (MLPA) revealed no causative mutation in this boy., The results of this study suggested that the single nucleotide polymorphisms of CREBBP do not influence diagnosis and treatment response in patients with major depressive disorder and bipolar disorder., We present the first evidence that the portion of E2A proteins found within the oncogenic fusion protein E2A-PBX1 and including the transcriptional activation domains can enhance the HAT activity of CBP/p300, Screening for mutations in the CREBBP gene from cases of high hyperdiploid (HD) childhood acute lymphoblastic leukemia resulted in an overall frequency of somatic CREBBP aberrations in HD ALL relapse cases of 63%., Overexpression of CBP/p300 in conjunction with E2A results in enhancement of E2A-induced histone acetylation, germline transcription, and immunoglobulin (Ig)kappa light chain rearrangement., Data indicate six new CBP mutations identified in Brazilian patients with Rubinstein-Taybi syndrome., The acetylase/deacetylase couple CREB-binding protein/Sirtuin 1 controls hypoxia-inducible factor 2 signaling., The study confirms that CREBBP is the critical gene involved in the duplication 16p13.3 syndrome., CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes., report of a Moroccan family with 2 children with Rubinstein-Taybi syndrome and apparently unaffected parents; molecular studies showed a heterozygous mutation c.4361T>A in exon 26 of the CREBBP gene in the affected siblings; neither parents, nor the healthy brother, carry this mutation in hematologic cells, Results show that Akt regulates acetyltransferase activity through CBP phosphorylation, which may contribute to tumorigenesis., HSV-1 VP16 is able to block the production of IFN-beta by inhibiting NF-kappaB p65 activation and interfering with IRF-3 to recruit its coactivator CBP, which may be important to the early events leading to HSV-1 infection., In the title., Characterize the structure of the transition state of the binding-induced folding in the reaction between the KIX domain of the CREB-binding protein and the transactivation domain of c-Myb., germline deletion in CREBBP gene is associated with Rubinstein-Taybi syndrome predisposing to non-WNT, non-SHH, group 3 medulloblastoma., Cryptic microdeletion of the CREBBP gene from t(1;16) (p36.2;p13.3) as a novel genetic defect causing Rubinstein-Taybi syndrome., Sp1 may regulate the expression of CD59, whereas CREB-binding protein (CBP)/p300 bridge NF-kappaB and CREB, which functions as an enhancer-binding protein to induce the up-regulation of CD59 during in lipopolysaccharide (LPS)-triggered complement activation., study defined eight additional recurrently mutated genes in SMZL; these genes are CREBBP, CBFA2T3, AMOTL1, FAT4, FBXO11, PLA2G4D, TRRAP and USH2A., provide evidence for the existence of a functional ternary complex containing TDG, CBP and activated RARalpha, our results suggest that capsaicin activated JNK and FOXO-1, leading to the acetylation of FOXO-1 through CBP and SirT-1, show how CBP and PTEN interact to mediate tumor suppression in the prostate, establishing a central role for histone modification in the etiology of prostate cancer, The combination of p300 and CBP expression may be useful in identifying patients with increased risks of cancer recurrence of small cell lung cancer., TDG, as a new coactivator, promotes beta-catenin/TCFs transactivation and functionally cooperates with CBP in canonical Wnt signaling., EP2/cAMP/protein kinase A pathway mediates the stimulatory effect of PGEs on angiogenesis essential for tissue injury healing via the induction of CREB activity and VEGF expression., First study of Korean Rubinstein-Taybi syndrome patients indicating distinct geographic distribution of CREBBP mutations, It is associated with episodic memory performance., CBP contributes to the upregulation of human telomerase reverse transcriptase expression and tumor growth, and overexpression of CBP predicts poor prognosis in human lung cancers., MacroH2A1.1 and PARP-1 cooperate to regulate transcription by promoting CBP-mediated H2B acetylation., 42 new CREBBP mutations were reported in 46 Rubinstein-Taybi syndrome patients., GR tau1c phosphorylation could promote CBP recruitment and enhance activation function 1 activity., destabilization of p300/CBP by downregulation of iASPP expression levels appears to represent a molecular mechanism that contributes to chemoresistance in melanoma cells., CREBBP mutation in follicular lymphoma progenitors is associated with decreased MHC class II expression., Characterization of 14 novel deletions underlying Rubinstein-Taybi syndrome, these data suggest that CBP/p300 are promising therapeutic targets across multiple subtypes in acute myeloid leukemia., CREBBP mutations are associated with recurrence in hyperdiploid acute lymphoblastic leukemia., Conclude that the CBP/beta-catenin complex is a core component of the MDR1 transcriptional "enhancesome" in neoplasms., WNT/beta-catenin signaling does not affect nuclear translocation of the RelA subunit of NF-kappaB or its association with CBP (also known as CREBBP), these findings suggest that sumoylation plays a critical role in the spatiotemporal co-activation of CLOCK-BMAL1 by CBP for immediate-early Per induction and the resetting of the circadian clock., Disruption of beta-catenin/CBP signaling inhibits human airway epithelial-mesenchymal transition and repair., RFPL3 and CBP have roles in upregulating hTERT activity and promoting lung cancer growth, Kaposi's sarcoma-associated herpesvirus vIRF4 targets the beta-catenin/CBP cofactor and blocks its occupancy on the cyclin D1 promoter, suppressing the G1-S cell cycle progression and enhancing virus replication., Case Report, Computational simulations were used to understand how phosphorylation affects the structure of the p53 terminal transactivation domain in complex with the CBP TAZ2 domain., Intrinsic protein disorder plays a prominent role in the function and interactions of the transcriptional co-activators CBP and p300. (Review),
OFFICIAL_GENE_SYMBOL CREBBP,
OMIM_DISEASE Rubinstein-Taybi syndrome,
SP_COMMENT catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone., disease:Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription., disease:Defects in CREBBP are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies., domain:The KIX domain mediates binding to HIV-1 Tat., function:Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes., online information:P300/CBP entry, PTM:Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., PTM:Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX., similarity:Contains 1 bromo domain., similarity:Contains 1 KIX domain., similarity:Contains 1 ZZ-type zinc finger., similarity:Contains 2 TAZ-type zinc fingers., subunit:Found in a complex containing NCOA2; NCOA3; IKKA; IKKB and IKBKG. Probably part of a complex with HIF1A and EP300. Interacts with phosphorylated CREB1. Interacts with the C-terminal region of CITED4. The TAZ-type 1 domain interacts with HIF1A. Interacts with MAF, SRCAP, CARM1, ELF3, MLLT7/FOXO4, N4BP2, NCOA1, NCOA3, NCOA6, PCAF, PELP1, PML, SMAD1, SMAD2, SMAD3, SPIB and TRERF1. Interacts with HTLV-1 Tax and p30II. Interacts with HIV-1 Tat. Interacts with KLF1; the interaction results in acetylation of KLF1 and enhancement of its transcriptional activity. Interacts with ZCCHC12 (By similarity). Interacts with DAXX; the interaction is dependent on CBP sumoylation and results in suppression of the transcriptional activiy via recruitment of HDAC2 to DAAX (By similarity). Interacts with MTDH. Interacts with NFATC4. Interacts with MAFG; the interaction acetylates MAFG in the basic region and stimulates NFE2 transcriptional activity through increasing its DNA-binding activity. Interacts with IRF2; the interaction acetylates IRF2 and regulates its activity on the H4 promoter.,
E1A binding protein p300(EP300) E1A binding protein p300(EP300) Related Genes Homo sapiens
CHROMOSOME 22,
CYTOBAND 22q13.2,
ENSEMBL_GENE_ID ENSG00000100393,
GENERIF_SUMMARY p300 stimulates Type I collagen synthesis in skin fibroblasts, p300 is involved in the antagonistic regulation of Type I collagen gene expression by Transforming Growth Factor-b and Interferon-g, substrate specificity; structure activity relationship, CBP/p300 and NcoA/SRC1a may function in a common pathway to regulate STAT3 transcriptional activity., p300 Modulates the BRCA1 inhibition of estrogen receptor activity, Scaffold/matrix attachment region elements interact with a p300-scaffold attachment factor A complex and are bound by acetylated nucleosomes., Results demonstrate that phosphorylation of p65 determines whether it associates with either CBP or HDAC-1, ensuring that only p65 entering the nucleus from cytoplasmic NF-kappa B, The HMG-I/Y-related protein p8 binds to p300 and Pax2 trans-activation domain-interacting protein to regulate the trans-activation activity of the Pax2A and Pax2B transcription factors on the glucagon gene promoter., Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with nucleosome assembly protein 1 in vitro., Carboxyl-terminal transactivation activity of hypoxia-inducible factor 1 alpha is governed by a von Hippel-Lindau protein-independent, hydroxylation-regulated association with p300/CBP., In vitro assays show an indispensable role for H3 and H4 tails, especially major lysine substrates, in p300-dependent transcriptional activation, as well as activator-targeted acetylation of promoter-proximal histone tails by p300., CBP-p300 plays a role in regulating p450scc expression with TReP-132 and steroidogenic factor-1, p300 and TATA-binding protein-free TAF-containing complex cooperate during transcriptional activation, p300 plays an important role in epithelial carcinogenesis by mediating transcription that negatively regulates cell proliferation.(P300-CBP coactivator), MDMX dramatically inhibits the acetylation of p53 induced by both endogenous and ectopically expressed p300/CBP., p300 coactivator has a role in suppressing tumor cell growth, results suggest that all-trans-retinoic acid and retinoic acid receptors regulate growth arrest of human mammary epithelial cells and modulate CBP/p300 protein expression, suggest an important role of p300 in regulation of HPV8 gene expression and reveal a new mechanism by which E2 may be recruited to a promoter to activate transcription without sequence specific DNA binding, PKC delta inhibits p300/CBP-associated factor activity after phosphorylation at serine 89, p300 plays a critical regulatory role in base excision repair, p300/CBP binds to IRF3, enabling it to act as a transcription factor, our results indicate an important role for Mediator, as well as its functional interplay with p300/CBP-SRC, in the enhancement of ERalpha-dependent transcription with chromatin templates, synergism with p68 RNA helicase, MAPK signaling facilitates HIF1a and HIF2a activation through p300/CBP, Interferon regulatory factor-7 synergizes with other transcription factors through multiple interactions with p300/CBP coactivators., in human mammary epithelial cells, CBP/p300 were both modulated by an all-trans-retinoic acid (ATRA) signaling pathway and were required for a normal response to ATRA, generation of the polyubiquitinated forms of p53 that are targeted for proteasome degradation requires the intrinsic ubiquitin ligase activities of MDM2 and p300, Loss of EP300 was not statistically significant selection in cancer cells stratified by various criteria for the concordant loss of EP300 and CREBBP., p300 may further regulate the transcriptional activity of p53 through a novel acetylation site, Lys-305, CBP and p300 function as co-activators of Sox9 for cartilage tissue-specific gene expression and chondrocyte differentiation., We investigated occupancy of ER-alpha promoter by pRb2/p130-E2F4/5-HDAC1-SUV39 H1-p300 and pRb2/p130-E2F4/5-HDAC1-SUV39H1-DNMT1 complexes, and provided a link between pRb2/p130 and chromatin-modifying enzymes in the regulation of ER-alpha transcription, S-nitrosation of Cys-800 stimulates the recruitment of p300 co-activator protein to the HIF-1alpha C-terminal domain, ER81 is acetylated by two coactivators/acetyltransferases, p300 and p300- and CBP-associated factor (P/CAF) . Whereas p300 acetylates two lysine residues (K33 and K116) within the ER81 N-terminal transactivation domain, P/CAF targets only K116., p300 plays a role in formation of the TBP-TFIIA-containing basal transcription complex, TAC., p300 has a role in NF-kappaB-dependent gene expression along with PARP-1, we show that E7 can abolish the p300-mediated E2 transactivation function, suggesting that complex formation between E7 and p300 may contribute to the regulation of E2 transcriptional activity., p300 has a role in the trans-repression of beta-catenin signaling by nuclear receptors and their ligands, EWS-ER81 and EWS-Fli-1 interact with and thereby activate the MMP-1 promoter, which is potentiated by the cofactor p300 and the proto-oncoprotein c-Jun., forms complex with SATB1 which binds to the 5' upstream AT-rich region in the bp -115 to bp -106 segment represses the gp91(phox) promoter activity, p300-mediated acetylation can be highly constrained by substrate conformation in vivo, p300 is the predominant coactivator that is essential for COX-2 transcriptional activation by proinflammatory mediators., Hypermethylation of p300 promoter is not an important mechanism in leukemogenesis, activity of ESE-1 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP., p300 markedly potentiates the binding of NFATc1 to the bcl-2 NFAT element by interacting with NFATc1 in an E1A-dependent manner., p300 is a key regulator of the p53 response and suggest that p300 inhibition could be used to modulate chemotherapy, Data show that RUNX3 is a target of the acetyltransferase activity of p300 acetyltransferase., a new insight into the molecular mechanisms by which the critical concentration of p300 protein is regulated and suggests a role for Akt in control of various cellular activities through the transcriptional coactivator p300, the IHD scaffold is an independent LXXLL peptide-binding domain within the p300 protein, complementing the known peptide-binding domains including IBiD, C/H1, and C/H3, Ectopically expressed p300 reverses the p53 mediated suppression of TGF-b-induced Type I collagen synthesis in fibroblasts., IRF-1-p300 interface as an allosteric modifier of DNA-dependent acetylation of p53 at the p21 promoter, The p300-CBP coactivator had a stabilizing effect on the formation of the complex between HIF-1alpha and its DNA-binding partner, Arnt., SIRT1 deacetylation and repression of p300 involves specific lysine residues in the cell cycle regulatory domain 1, Both TAZ domains of CREB-binding proteins CBP and p300 in the presence of stoichiometric concentrations of zinc adopt a well-defined structure in solution in the absence of binding partners., Activation of Stat3 sequence-specific DNA binding and transcription by p300/CREB-binding protein-mediated acetylation., p300 is essential for SENP1 to enhance c-Jun-dependent transcription because SENP1 can desumoylate the CRD1 domain of p300, thereby releasing the cis-repression of CRD1 on p300, Transfection of p300 into prostate cancer cells specifically increases mRNA and protein levels of nuclear matrix peptides lamins A and C., We extended the search for mutations to the EP300 gene and showed that mutations in EP300 also cause this disorder. These are the first mutations identified in EP300 for a congenital disorder., A novel and unique PAX8 mutation provides evidence for a crucial role of the p300 coactivator in mediating the functional synergism between PAX8 and TTF-1 in thyroid-specific gene expression., TCF4 expression mediated by beta-catenin/p300 may be important for initial steps during trans-differentiation of endometrial carcinoma cells., p300 directly acetylates lysines in the carboxyl-terminal region of Foxo1 in vivo and in vitro, and potently stimulates Foxo1-induced transcription of IGF-binding protein-1., We report a novel, noncompetitive mechanism linking acetylation and ubiquitination, in which the association of transcription factor E2F-1 with the cellular coactivator and acetyltransferase p300 determines its acetylation and subsequent ubiquitination., data indicate that p12I appears to play a vital role in HTLV-1-mediated T cell activation by activating calcium-mediated transcription and augmenting the amounts of p300 within T lymphocytes, cyclin D1 plays an important role in cellular proliferation and differentiation through regulation of p300., Histone acetyltransferase activity of p300 is required for transcriptional repression by PZLF., p300 regulates p63-dependent transcription of p21, Sox9 and p300 interact with chromatin and activates transcription via regulation of chromatin modification, both p300 and CBP increase ATF4 transcriptional activity, point mutations are uncommon in patients with myeloproliferative disorders, GABPA and EP300 are essential components of a retinoic acid-induced enhanceosome in myeloid cells., In cells transfected with both IRF-2 and p300/CBP-associated factor , IRF-2 associated with endogenous nucleolin., loss of p300 in HCT116 cells results in potentially aggressive phenotype characterized by reduced adhesion and increased migration, Data shpw that SII is a major component of chromatin transcription and strongly synergizes with p300 (histone acetylation) at a step subsequent to preinitiation complex formation., Data show that nuclear accumulations of p53 and Mdm2 are accompanied by reductions in c-Abl and p300 in zinc-depleted human hepatoblastoma cells., Role for p300 in promoting cell survival, which is independent of its acetyltransferase activity and acts at least in part through FGF-1., Tat-mediated activation of the HIV promoter requires the SWI/SNF complex in synergy with the coactivator p300., DEK interacts with histones and exerts a potent inhibitory effect on both p300 and PCAF-mediated histone acetyltransferase activity and transcription., PPARGC1A, PPARGC1B, and EP300 may have roles for familial breast cancer susceptibility, the transcription factor p300 is a potential repressor of telomerase activation and histone acetylation in the context of E6 expression, Levels of expression of CtBP and p300 are critical for the action of SNAIL and ZEB1, which have a pivotal role in levels of epithelial-mesenchymsal transitionin human colon carcinoma., coordinated binding of Zac zinc fingers and C terminus to p300 regulates HAT function by increasing histone and acetyl coenzyme A affinities and catalytic activity, HDAC4 (histone deacetylase 4) and p300, a known HAT (histone acetyltransferase), reversibly controlled the activity of the IL-5 promoter in vivo and in vitro, with a concurrent alteration of histone H3 acetylation status at the promoter regions., Transcriptional coactivator p300 may regulate fibronectin expression via PARP and NF-kappaB activation in diabetes., hypothesized that HSV-1 ICP0 recruits activated IRF-3 & CBP/p300 to nuclear structures, away from host chromatin; this leads to inactivation & accelerated degradation of IRF-3, resulting in reduced transcription of IFN-beta & inhibition of host response, The patients with EP300 mutations displayed the typical facial gestalt and malformation pattern compatible with the diagnosis of Rubinstein-Taybi syndrome., p72 RNA helicase may not only be involved in the p53-Mdm2 regulatory loop, but also profoundly impact on the transcriptome through various CBP/p300 and P/CAF interacting proteins., MITF evokes transcription of a paradigmatic MITF target tyrosinase and show that the adenoviral E1A protein represses the MITF-driven transcription in these cells., Role for p300 acetyltransferase includes the modulation of chromatin structure and function during DNA metabolic events as well as for transcription., EP300 mutations could be underdiagnosed because they may result in phenotypes different from classical Rubinstein-Taybi syndrome., Asparagine hydroxylation and S-nitrosylation of HIF-1alpha decrease p300 binding, while its phosphorylation does not affect p300 binding, which was reconfirmed by competitive inhibition analyses using mutant peptides., p300-mediated acetylation of p53 requires Bat3, CBP/p300 has an evolutionarily conserved role as a buffer regulating TCF-beta-cat/Arm binding., A complex between tetrameric p53 and p300 in which four domains of p300 wrap around the four transactivation domains of p53., These results indicate that impaired competition with p300 is the possible cause of dysfunction of c-Ski/SnoN in scleroderma fibroblasts and that this might contribute to maintenance of the autocrine TGFbeta loop in this disease., ERK2-mediated C-terminal serine phosphorylation of p300 was a key event in the regulation of EGF-induced keratin 16 expression., These results provide evidence for a model in which Tax and pCREB bind distinct surfaces of KIX for effective CBP/p300 recruitment to the HTLV-1 promoter., demonstrate the distinct regulatory mechanisms of p300 and TR3 on retinoid X receptor-alpha acetylation, Data show that there are combinatorial effects of CBP/p300, Ets2, and PKA triggered via growth factors released from maternal endometrium., These data reveal that phosphorylation of CREB is not sufficient for CBP/p300 recruitment and transcriptional activation., PGE(2) via EP(2) and EP(4) activates the cAMP-->PKA-->CREB pathway leading to enhanced CYP19 transcription and increased aromatase activity through BRCA1 and p300, that Skp2 controls p300-p53 signaling pathways in cancer cells, making Skp2 a potential molecular target for cancer therapy., p300/HAT activation has a role as a very early response to shear that is essential for increasing eNOS mRNA levels, p300 can serve as a scaffold for the E1A repression domain to access specific cellular gene promoters involved in growth regulation., high levels of p300 and C/EBPalpha in suprabasal layers of HPV16 positive cervical lesions allow the viral E2 protein to activate HPV16 early gene expression, C-terminal region of p300 provides corepressor function and facilitates the recruitment of p300 and HDAC3 to the YY1-binding site and represses the c-Myc promoter., A long unstructured chain (or "flap") overlaying the acetyl-CoA binding groove, and a four-alpha-helix "tower" excursion from the main beta-sheet--critically contribute to the recognition and presumptive catalytic machinery of p300., Results identify TFIIIC as a recruitment factor for p300 and thus may have important implications for the emerging concept that tRNA genes or TFIIIC binding sites act as chromatin barriers to prohibit spreading of silenced heterochromatin domains., These observations demonstrate that p300 can inactivate Sirt2 by acetylation and that p300 may regulate the activity of p53 indirectly through Sirt2 in addition to its direct modification of p53., lower transactivity of PPAR delta for arachidonic acid in Caco-2 cells, compared with PPAR alpha, is associated with the binding activity of p300 to the receptor, Fbx3 formed SCF(Fbx3) ubiquitin ligase and promoted the degradation of HIPK2 and p300 by the ubiquitin-proteasome pathway; PML inhibited this degradation, p300 undergoes a dynamic cycle of autoacetylation and deacetylation regulated by SIRT2., Brucella controls host defenses by impairing macrophage responses to IFNgamma induced STAT1-CBP/P300 association., functional inactivation of HIF-alpha by stimulation of FIH-dependent p300 contributes to the YC-1-induced deregulation of hypoxia-induced genes, p300 histone acetyltransferase, one of the NFkappaB-associated coactivators, reactivates epigenetically silenced MLH1 in 293T cells., The effects of specific p53 phosphorylations on its interaction with the Taz2 domain of p300, are examined., Estrogen increases transcription in a rapid but transient manner at early estrogen-repressed genes but that this is followed by recruitment of the corepressor CtBP1, a p300-interacting partner that plays an essential role in the repressive process., The authors demonstrate that the nuclei of Tax-expressing cells, including HTLV-1 transformed T-lymphocytes, contain a pool of Tax molecules acetylated on lysine residue at amino acid position 346 by the transcriptional coactivator p300., STAT3 transactivates its target genes by the recruitment of CBP/p300 co-activators and this process generally does not require the contribution of SRC-1., Thermodynamic experiments revealed the importance of hydrophobic interactions in the complex of Taz2 with p53 phosphorylated at Ser(15) and Thr(18)., demonstrate that the histone acetyl transferase CBP (also known as Nejire) in flies and CBP and p300 (Ep300) in humans acetylate H3K56, Data show that acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 and that acetylation functions in concert with Keap1-mediated ubiquitination in modulating the Nrf2-dependent antioxidant response., p21(WAF1)-p300-DNMT1 pathway may play a pivotal role to ensure regulated DNMT1 expression and DNA methylation in mammalian cell division., NF-kappaB and p300 are involved in the expression of vascular cell adhesion molecule-1 induced by interleukin-1beta in human tracheal smooth muscle cells., p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization., These results point to an important role for p300 in maintaining genomic integrity by negatively regulating c-myc., p300 can inactivate HDAC6 by acetylation; p300 may regulate the activity of Sp1 indirectly through HDAC6 in addition to its direct modification of Sp1., results indicate that PPAR-gamma blocked Smad-mediated transcriptional responses by preventing p300 recruitment and histone H4 hyperacetylation, resulting in the inhibition of TGF-beta-induced collagen gene expression, The authors show that ORF3-mediated extracellularly regulated kinase (Erk) activation was responsible for the observed increase in phosphorylation and transactivation activity of p300/CBP., Oveexpression of p300 upregulated hPTTG at the levels of promoter activity, mRNA and protein expression., epigenetic alterations with upregulated EP300 targeting miRNAs miR-194, miR-200b, miR-200c and miR-429 are related to reduced EP300 mRNA and protein in pancreatic ductal adenocarcinoma, RTK1 specifically inhibits the p300-mediated acetylation of p53 but not the acetylation by another acetyltransferase, p300/CREB-binding protein -associated factor, SENP3 is a redox sensor that regulates HIF-1 transcriptional activity under oxidative stress through the de-SUMOylation of p300, Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3., Data show that cytoplasmic p300/CBP E4 activities ubiquitinate and destabilize p53, while physically separate nuclear p300/CBP activities, such as p53 acetylation, activate p53., Data support a model in which Notch-1 can activate the transcription of ERalpha-target genes via IKKalpha-dependent cooperative chromatin recruitment of Notch-CSL-MAML1 and ERalpha, which promotes the recruitment of p300., CBP is the coactivator directly interacting with HIF-1 alpha N-TAD and mediating the transactivation function of this domain in hypoxia, These findings suggest that p300 is likely to play an important role in biological processes in which transcriptional bookmarking or preconditioning influences cellular growth and development., PGI(2) exerts anti-inflammatory action by inhibiting STAT1-induced cytokine production, in part by targeting the transactivation domain-induced recruitment of the histone acetylase CBP/p300., structure of p300 comprises an atypical helical bundle stabilized by three Zn ions and closely resembles the solution structures determined previously for shorter peptides., propose that individuals with EP300 mutations may exhibit a slightly different phenotype compared to individuals with CREBBP mutations, Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates., These results suggest that in human tracheal smooth muscle cells, activation of MAPKs, NF-kappaB, and p300 are essential for IL-1beta-induced cPLA(2) expression and PGE(2) secretion., Cdk9 forms a functional complex with the p300/GATA4 and is required for p300/GATA4- transcriptional pathway during cardiomyocyte hypertrophy, Tax binds the cyclin D1 promoter-proximal cyclic AMP response element (CRE) in the presence of phosphorylated CREB (pCREB) in vitro, and together the Tax-pCREB complex recruits the cellular co-activator p300 to the promoter., p300 plays a central coactivator role in hypoxic induction of EPO, ). miR-132 regulates innate antiviral immunity by inhibiting expression of the p300 transcriptional co-activator; p300 regulates miR-132 levels, revealing a dynamic equilibrium between miR-132 and p300, p300/HDAC1-induced dynamic changes in the acetylation of Nur77 protein play an important role in the control of Nur77 protein turnover., MAGE-11 links NH(2)-terminal domains of AR and p300 to promote transcriptional synergy through a cadre of FXXLF-related interacting motifs, These data demonstrate that ATM transduces a DNA damage signal to p300, and that ATM-dependent phosphorylation of p300 is required for stabilization of NBS1 proteins in response to DNA damage., The bromodomain is important for p300 to maintain the basal level of histone acetylation and to induce the transcriptional activation of p300-dependent genes., p300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammals., Using a patient-derived cell line, the authors show that p300 sequestration into the chromatin-bound BRD4-NUT fusion protein foci is the principal oncogenic mechanism leading to p53 inactivation., Sp1-mediated RASSF2A gene transcription is activated by p300 through histone acetylation, and this activation plays an important role in inducing late apoptosis., Exon coverage microarray platform detected intragenic deletions, Data show that activator-dependent p300 histone acetylation disrupted both inter- and intrafiber nucleosome-nucleosome interactions and simultaneously led to enhanced RNAPII transcription from the decondensed model chromatin., Our results reveal an essential role of p300-mediated acetylation in regulating the stability of ThPOK, p300 and CBP are implicated in regulation of ER-beta activity and cellular migration in prostate cancer., BCL6 repression of EP300 in human diffuse large B cell lymphoma cells provides a basis for rational combinatorial therapy., Deletion of CBP/p300 specifically and dramatically reduces acetylations on H3K18 and H3K27., transcriptional coactivator p300 may be partially degraded during SMC differentiation, leaving an activated subpopulation with increased HAT activity and SMC differentiation-gene specificity, NF-kappaB-mediated inflammatory signaling is able to block HIF-1alpha transactivation at hypoxia response elements-encoding genes by direct competition for p300 binding at the promoter., Ep300 overexpression is an independent biomarker for poor prognosis of patients with hepatocellular carcinoma., Data show that knock down of p300DeltaC in RC-K8 cells reduced their growth in both liquid culture and soft agar., Recruitment of BRCA1 to the p53-binding region of the p21 promoter in response to DNA damage required methylation of Arg 754 of p300 by CARM1, Cigarette smoke extract regulates cytosolic phospholipase A2 expression via NADPH oxidase/MAPKs/AP-1 and p300 in human tracheal smooth muscle cells., findings show that the transcriptional co-activator p300 was expressed in glioblastoma multiforme tumors and cell lines and acted as an activator of the GFAP gene and a repressor of the Nestin gene, p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity, results identify CREBBP/EP300 mutations as a major pathogenetic mechanism shared by common forms of B-cell non-Hodgkin's lymphoma, with direct implications for the use of drugs targeting acetylation/deacetylation mechanisms, Single Nucleotide Polymorphisms in EP300 is associated with childhood acute lymphoblastic leukemia., Apoptosis Stimulating Proteins of p53 (ASPP) proteins can function together with p300 in regulating the transcriptional activity of p53, High expression of EP300 protein human, suggest poor prognosis for patients with resectable esophageal squamous cell carcinoma., ERbeta and its co-regulators p300 and NCoR are expressed in human transitional cell bladder cancer, Adenosine monophosphate-activated protein kinase (AMPK) inhibits transforming growth factor (TGF)-beta-induced fibrogenic property of activated hepatic stellate cells by regulating transcriptional coactivator p300., Knocking down of p300 expression in hepatoma cells recovered E-cadherin expression, inhibited the translocation of beta (beta)-catenin into the nuclei, decreased cyclin D1 activity and suppressed the migration/invasion of HCC cells., study found AML1-ETO is acetylated by the transcriptional coactivator p300 in leukemia cells isolated from t(8;21) AML patients, and this acetylation is essential for its self-renewal-promoting effects in cord blood CD34(+) cells and its leukemogenicity, CBP/p300 and SIRT1 were recruited to histone gene promoters in an NPAT-dependent manner, knockdown of which affected histone acetylation on histone gene promoters and histone gene transcription., These fi ndings assign a central role for the ATF5/p300 complex in ATF5 function., The authors have identified the acetyltransferase p300 as an additional target of Escherichia coli type III secreted protease NleC., Results indicate that quercetin suppresses COX-2 expression by inhibiting the p300 signaling and blocking the binding of multiple transactivators to COX-2 promoter., Beta-HPV 5 and 8 E6 degrade p300 in a proteasomal-dependent, E6AP-independent manner. E6 competes with AKT for binding to p300., p300 acetylates Notch1 ICD in cell culture assay and in vitro, and conserved lysines located within the Notch C-terminal nuclear localization signal are essential for Notch acetylation., Transcriptional repression mediated by IER5 regulates Cdc25B expression levels via the release of NF-YB and p300 in acute myeloid leukemia., CREBBP and EP300 mutations are not major pathogenetic mechanisms of myeloproliferative neoplasms, The difference in the spatiotemporal expression of p300 and CBP indicated that they play different roles in tooth development., our findings show that the E2A proteins are acetylated at specific residues as a consequence of lysine acetyltranferases(CBP, p300 and PCAF ) recruitment, Evaluated the influence of gene variant A>G rs20551 in EP300 in donor and/or recipient on clinical results after HLA-identical sibling allo-SCT., A hitherto unreported but paternally inherited heterozygous sequence alteration, c.506 1+9C>T in IVS 30-31, which most likely represents a normal variant (NetGene 2 splice prediction software)., p300 and PCAF cooperate in the control of microRNA 200c/141 transcription and epithelial characteristics, We present the first evidence that the portion of E2A proteins found within the oncogenic fusion protein E2A-PBX1 and including the transcriptional activation domains can enhance the HAT activity of CBP/p300, Low expression of p300 is an independent prognostic marker of better survival in operable NSCLC patients., Data show that the interaction between FOXO3a and CBP/p300 is indicated by additional binding sites on CBP/p300., Overexpression of transcription factors CBP/p300 in conjunction with E2A results in enhancement of E2A-induced histone acetylation, germline transcription, and immunoglobulin (Ig)kappa light chain rearrangement., variants of the transcriptional coactivator genes (EP300 and PCAF) may influence Hepatocellular carcinoma risk in populations with low mutations or chromosomal instability rates., Studies provide a mechanism whereby acetylation of Skp2 by p300 regulates protein stability and cellular localization, thus modulating oncogenic signaling., A genome-wide association study on a southern European population identified a new Crohn's disease susceptibility locus at RBX1-EP300., studies suggest that the HPV16-E7 and Ad5-E1A viral oncoproteins bind to the same p300 CH1 domain to disrupt p300 function by distinct mechanisms., GR and p300 are involved in the induction of H6PD by cortisol in human amnion fibroblasts, the intrinsically disordered region of the acetyltransferase p300 with similarity to prion-like domains plays a role in aggregation, A novel upstream role for miR-150 in p300-mediated cardiomyocyte hypertrophy., Data indicate that mutation of E3 ligase motif not only abolishes BRMS1-induced p300 polyubiquitination and degradation, but importantly, dramatically reduces the metastasis suppressor function of BRMS1., CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes., To best of our knowledge, we provide first evidence that GNPs potentiate cell death by activating p300/CBP histone acetyltransferases., p300 associates with the RLIP76 promoter via an overlapping cMYB and cETS binding site and regulates RLIP76 promoter activity and its expression., a stress p53-Mdm2-p300-HDAC1 regulatory network that is activated and stabilised by two regulatory proteins, p300 and HDAC1, is reported., cAMP signalling decreases the level of the p300 protein by promoting its ubiquitin-proteasome dependent degradation, which is mediated by Epac and p38 MAPK, TTC5 and EP300 cooperate to prevent excessive accumulation of MYC in AML cells and their sensitization to cell death., Data indicate that IKKbeta inhibited the binding between TAp63gamma and p300, a co-activator of TAp63gamma, and consequently counteracted the positive effect of p300 on TAp63gamma transcriptional activity., Data indicate that menadione (MD) and ethacrynic acid (EA) decrease expression of luciferase under the control of hypoxia-responsive elements in hypoxic cells as well as to efficiently block the interaction between the full-length HIF-1alpha and p300., Levels of p300, PKCbeta2 and ROS in HG and AGEs groups were elevated by 1.04, 1.26, 0.78 and 1.45, 1.07, 0.71 folds of those in NG group., p300 contributed to both IFN-gamma- and IRF-1-mediated TRIM22 transcription independent of its histone acetyltransferase activity, however, it was required for the recruitment of RNA polymerase II to TRIM22 promoter region., Frameshift mutations of tumor suppressor gene EP300 occur in gastric and colorectal cancers with high microsatellite instability., Charge density distribution and the electrostatic moments of CTPB in the active site of p300 enzyme, CaMKK/Akt/p300 cascade plays an important role in laminar stress-dependent induction of KCa2.3 and KCa3.1 expression, thereby regulating EC function and adaptation to hemodynamic changes., S100A6 and the TAZ2 domain of p300 bind p53 with similar affinities and S100A6 effectively competes with TAZ2 for binding to p53., These results establish a mechanism in which SET1 complex and p300 act cooperatively, through direct interactions and coupled histone modifications, to facilitate the function of p53., Authors identified acetyltransferase p300 and deacetylase HDAC7 as enzymes modulating human T cell leukemia virus type 1 Tax protein acetylation., Present the 2.8-A crystal structure of the catalytic core of p300 containing its bromodomain, CH2 region and HAT domain. The structure reveals that the CH2 region contains a discontinuous PHD domain interrupted by a RING domain., we explored how these HIF-specific hydroxylases contribute to the HIF-1alpha-p300 interaction by FRET measurements along with HIF-1 mediated transcriptional activation, transcriptional associations with HIF-1alpha and NF-kappaB, as well as beta-catenin/p300 complexes, may contribute to modulation of changes in tumor cell kinetics in response to a hypoxic condition in endometrial carcinoma cells, these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation., Data suggest that megakaryoblastic leukemia 1 (MKL1) and histone acetyltransferase p300 could augment the expression of catechol-O-methyltransferase (COMT), increase estrogen metabolism, and thus reduce MCF-7 cell proliferation stimulated by estrogen., Data suggest that SMYD2-mediated estrogen receptor alpha (ERalpha) protein methylation and p300/cAMP response element-binding protein-binding protein-dependent ERalpha acetylation play an important role in the estrogen-induced gene expression profiles., Immunohistochemical p300 expression is significantly associated with histology and lymph node involvement in colorectal cancer, and overexpression may be an independent favorable prognostic factor for disease-free survival., P300 drives an angiogenic transcription program during hypertrophy that is fine-tuned in part through direct repression of p300 by miR-20a., Findings demonstrate that CypB prevents hypoxia-induced cell death through modulation of ubiquitin-mediated CHOP protein degradation, suggesting that CypB may have an important role in the tight regulation of CHOP under hypoxia., 25 cluster by targeting a transcriptional activator of E-cadherin., Sp1 may regulate the expression of CD59, whereas CREB-binding protein (CBP)/p300 bridge NF-kappaB and CREB, which functions as an enhancer-binding protein to induce the up-regulation of CD59 during in lipopolysaccharide (LPS)-triggered complement activation., results reveal a novel post-translational regulation of MTA2 by the way of p300-dependent acetylation, which is important for tumor cells growth and migration and provides a potential target for clinical cancer research, KAT3B acetyltransferase activity inhibition by 1,4-naphthoquinones involves redox cycling and nucleophilic adduct formation, this study indicates that EP300-related Rubinstein-Taybi syndrome cases occur more frequently than previously thought ( 8% vs 3-5%), Phosphorylation of p300 increases its protein degradation to enhance the lung cancer progression., The combination of p300 and CBP expression may be useful in identifying patients with increased risks of cancer recurrence of small cell lung cancer., Based on analysis, HSF1 activation upon heat shock involves the function of multiple chromatin modifiers, centering on the EP300/CREBBP histone acetyltransferase complex. Silencing of EP300 destabilizes HSF1 in nonstressed cells and induces its proteasomal degradation., A complex comprising p300/c-Jun/PARP-1 that bound wild type., Factor acetyltransferase p300 (FATp300), a major epigenetic regulator that acetylates specific lysines in histones and transcription factors, is essential for elevated collagen synthesis and the levels of FATp300 are significantly elevated in different fibrotic tissues., EDAG forms complex with GATA1 and p300 and increases GATA1 acetylation and transcriptional activity by facilitating the interaction between GATA1 and p300, Our results indicate a cross-talk between Braf and p300 in melanoma and demonstrate the importance Braf and p300 expression in the diagnosis and prognosis of melanoma., Promoter-bound p300 complexes facilitate post-mitotic transmission of transcriptional memory., Results show that p300 plays an important role in regulating the expression of key pluripotency genes in dental pulp cells and modifying odontogenic differentiation., Study tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternative EDB exon; these data suggest that histone acetylation could be one of the mechanisms how promoter and promoter binding proteins influence alternative splicing., estrogen receptor binding and p300 redistribution have a role in a squelching mechanism for estradiol-repressed genes, p300 overexpression and its association with clinical factors in patients with OSCC, it is likely that p300 itself or one of its target genes plays a key role in the aggressive phenotypes of OSCC., Data indicate that eletion of the PHD finger impairs histone acetylation by p300 protein., the presence of p300 in the cytoplasm correlates with increased survival of breast cancer patients, MiR-630 reduced apoptosis by downregulating several apoptotic modulators, PARP3, DDIT4, and EP300., P28(GANK) interacted with p300 to attenuate assembly of RelA with p300., this work has broad implications beyond myeloma because shed syndecan-1 is present in high levels in many tumor types as well as in other disease states., Data indicate that HPV16E7, CBP/p300, and retinoblastoma protein pRb interactions are potentially important for cellular transformation., destabilization of p300/CBP by downregulation of iASPP expression levels appears to represent a molecular mechanism that contributes to chemoresistance in melanoma cells., Our patient expands the phenotypic spectrum in EP300-related Rubinstein-Taybi syndrome; this case demonstrates the evolving practice of clinical genomics related to increasing availability of genomic sequencing methods., ER-alpha, SRC-3, and p300 form an active protein complex on estrogen response element-DNA., Data show that Naphthol AS-E phosphate inhibits proto-oncogene protein Myb activity by disrupting its interaction with E1A binding protein p300., MRTF-A and p300 activated the transcription of VE-cadherin gene by enhancing acetylation of histones., ZBRK1 suppresses renal cancer progression perhaps by regulating VHL expression through formation of a complex with VHL and p300 in renal cancer, Data from studies using peptide fragments suggest that complexes formed by the two transcriptional subdomains of TP53 (tumor protein p53) with EP300 exhibit different conformations and play critical roles in p53 transcriptional activity., Results show that the coactivator p300 has both crotonyltransferase and acetyltransferase activities, and that p300-catalyzed histone crotonylation directly stimulates transcription to a greater degree than histone acetylation., DDX24 inhibits p300 mediated acetylation of p53., these data suggest that CBP/p300 are promising therapeutic targets across multiple subtypes in acute myeloid leukemia., Our results link FGF8, c-Abl and p300 in a regulatory pathway that controls DeltaNp63alpha protein stability and transcriptional activity., the histone methyltransferase core enzyme ASH2L was bound at EGFR in the germinal matrix and in gliomas where levels of H3K4me3 are high, and the histone acetyltransferase P300 was bound in samples with H3K27ac enrichment, The Tax oncogene enhances ELL incorporation into p300 and P-TEFb containing protein complexes to activate transcription., DOT1L epigenetically derepresses epithelial-mesenchymal transition transcription factors with c-Myc and p300., levels of p300 protein are temporally maintained in ligand-enhanced skeletal myocyte development. this maintenance of p300 protein is observed at the stage of myoblast differentiation, which coincides with an increase in Akt phosphorylation., p300 and MRTF-A synergistically enhance the expression of migration-related genes in breast cancer cells., these results demonstrate that an interaction between STAT1, p300, and peroxisome proliferator-activated receptor-gamma is required for 15(S)-HETE-induced CD36 expression, oxidized low density lipoprotein uptake, and foam cell formation, RORgammat is acetylated, and this acetylation is reciprocally regulated by the histone acetyltransferase p300 and the histone deacetylase HDAC1., coactivator p300 mediates cytokine-induced hiNOS transactivation by forming a distant DNA loop between its enhancer and core promoter region, Sox4 was also able to promote b-catenin-mediated transcription of the Slug gene through formation of transcriptional complexes with b-catenin and p300, independent of TCF4 status, PIAS1 enhances p300 recruitment to c-Myb-bound sites through interaction with both proteins. In addition, the E3 activity of PIAS1 enhances further its coactivation,
OFFICIAL_GENE_SYMBOL EP300,
OMIM_DISEASE Colorectal cancer, somatic, Rubinstein-Taybi syndrome 2,
SP_COMMENT catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone., disease:Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3., disease:Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies., disease:Defects in EP300 may play a role in epithelial cancer., function:Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes., online information:P300/CBP entry, PTM:Acetylated on Lys at up to 17 positions by intermolecular autocatalysis., PTM:Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1., PTM:Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1., PTM:Phosphorylated., similarity:Contains 1 bromo domain., similarity:Contains 1 KIX domain., similarity:Contains 1 ZZ-type zinc finger., similarity:Contains 2 TAZ-type zinc fingers., subunit:Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, PRDM6, SPIB, SRY, TCF7L2, TP53, SRCAP, TTC5, JMY and TRERF1. The TAZ-type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with ING5. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat.,