Database for Annotation, Visualization and Integrated Discovery 2.1
National Institute of Allergy and Infectious Disease
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E1A binding protein p300(EP300) E1A binding protein p300(EP300) Related Genes Homo sapiens
CYTOBAND 22q13.2,
GENERIF_SUMMARY p300 stimulates Type I collagen synthesis in skin fibroblasts, p300 is involved in the antagonistic regulation of Type I collagen gene expression by Transforming Growth Factor-b and Interferon-g, substrate specificity; structure activity relationship, CBP/p300 and NcoA/SRC1a may function in a common pathway to regulate STAT3 transcriptional activity., p300 Modulates the BRCA1 inhibition of estrogen receptor activity, Scaffold/matrix attachment region elements interact with a p300-scaffold attachment factor A complex and are bound by acetylated nucleosomes., Results demonstrate that phosphorylation of p65 determines whether it associates with either CBP or HDAC-1, ensuring that only p65 entering the nucleus from cytoplasmic NF-kappa B, The HMG-I/Y-related protein p8 binds to p300 and Pax2 trans-activation domain-interacting protein to regulate the trans-activation activity of the Pax2A and Pax2B transcription factors on the glucagon gene promoter., Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with nucleosome assembly protein 1 in vitro., Carboxyl-terminal transactivation activity of hypoxia-inducible factor 1 alpha is governed by a von Hippel-Lindau protein-independent, hydroxylation-regulated association with p300/CBP., In vitro assays show an indispensable role for H3 and H4 tails, especially major lysine substrates, in p300-dependent transcriptional activation, as well as activator-targeted acetylation of promoter-proximal histone tails by p300., CBP-p300 plays a role in regulating p450scc expression with TReP-132 and steroidogenic factor-1, p300 and TATA-binding protein-free TAF-containing complex cooperate during transcriptional activation, p300 plays an important role in epithelial carcinogenesis by mediating transcription that negatively regulates cell proliferation.(P300-CBP coactivator), MDMX dramatically inhibits the acetylation of p53 induced by both endogenous and ectopically expressed p300/CBP., p300 coactivator has a role in suppressing tumor cell growth, results suggest that all-trans-retinoic acid and retinoic acid receptors regulate growth arrest of human mammary epithelial cells and modulate CBP/p300 protein expression, suggest an important role of p300 in regulation of HPV8 gene expression and reveal a new mechanism by which E2 may be recruited to a promoter to activate transcription without sequence specific DNA binding, PKC delta inhibits p300/CBP-associated factor activity after phosphorylation at serine 89, p300 plays a critical regulatory role in base excision repair, p300/CBP binds to IRF3, enabling it to act as a transcription factor, our results indicate an important role for Mediator, as well as its functional interplay with p300/CBP-SRC, in the enhancement of ERalpha-dependent transcription with chromatin templates, synergism with p68 RNA helicase, MAPK signaling facilitates HIF1a and HIF2a activation through p300/CBP, Interferon regulatory factor-7 synergizes with other transcription factors through multiple interactions with p300/CBP coactivators., in human mammary epithelial cells, CBP/p300 were both modulated by an all-trans-retinoic acid (ATRA) signaling pathway and were required for a normal response to ATRA, generation of the polyubiquitinated forms of p53 that are targeted for proteasome degradation requires the intrinsic ubiquitin ligase activities of MDM2 and p300, Loss of EP300 was not statistically significant selection in cancer cells stratified by various criteria for the concordant loss of EP300 and CREBBP., p300 may further regulate the transcriptional activity of p53 through a novel acetylation site, Lys-305, CBP and p300 function as co-activators of Sox9 for cartilage tissue-specific gene expression and chondrocyte differentiation., We investigated occupancy of ER-alpha promoter by pRb2/p130-E2F4/5-HDAC1-SUV39 H1-p300 and pRb2/p130-E2F4/5-HDAC1-SUV39H1-DNMT1 complexes, and provided a link between pRb2/p130 and chromatin-modifying enzymes in the regulation of ER-alpha transcription, S-nitrosation of Cys-800 stimulates the recruitment of p300 co-activator protein to the HIF-1alpha C-terminal domain, ER81 is acetylated by two coactivators/acetyltransferases, p300 and p300- and CBP-associated factor (P/CAF) . Whereas p300 acetylates two lysine residues (K33 and K116) within the ER81 N-terminal transactivation domain, P/CAF targets only K116., p300 plays a role in formation of the TBP-TFIIA-containing basal transcription complex, TAC., p300 has a role in NF-kappaB-dependent gene expression along with PARP-1, we show that E7 can abolish the p300-mediated E2 transactivation function, suggesting that complex formation between E7 and p300 may contribute to the regulation of E2 transcriptional activity., p300 has a role in the trans-repression of beta-catenin signaling by nuclear receptors and their ligands, EWS-ER81 and EWS-Fli-1 interact with and thereby activate the MMP-1 promoter, which is potentiated by the cofactor p300 and the proto-oncoprotein c-Jun., forms complex with SATB1 which binds to the 5' upstream AT-rich region in the bp -115 to bp -106 segment represses the gp91(phox) promoter activity, p300-mediated acetylation can be highly constrained by substrate conformation in vivo, p300 is the predominant coactivator that is essential for COX-2 transcriptional activation by proinflammatory mediators., Hypermethylation of p300 promoter is not an important mechanism in leukemogenesis, activity of ESE-1 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP., p300 markedly potentiates the binding of NFATc1 to the bcl-2 NFAT element by interacting with NFATc1 in an E1A-dependent manner., p300 is a key regulator of the p53 response and suggest that p300 inhibition could be used to modulate chemotherapy, Data show that RUNX3 is a target of the acetyltransferase activity of p300 acetyltransferase., a new insight into the molecular mechanisms by which the critical concentration of p300 protein is regulated and suggests a role for Akt in control of various cellular activities through the transcriptional coactivator p300, the IHD scaffold is an independent LXXLL peptide-binding domain within the p300 protein, complementing the known peptide-binding domains including IBiD, C/H1, and C/H3, Ectopically expressed p300 reverses the p53 mediated suppression of TGF-b-induced Type I collagen synthesis in fibroblasts., IRF-1-p300 interface as an allosteric modifier of DNA-dependent acetylation of p53 at the p21 promoter, The p300-CBP coactivator had a stabilizing effect on the formation of the complex between HIF-1alpha and its DNA-binding partner, Arnt., SIRT1 deacetylation and repression of p300 involves specific lysine residues in the cell cycle regulatory domain 1, Both TAZ domains of CREB-binding proteins CBP and p300 in the presence of stoichiometric concentrations of zinc adopt a well-defined structure in solution in the absence of binding partners., Activation of Stat3 sequence-specific DNA binding and transcription by p300/CREB-binding protein-mediated acetylation., p300 is essential for SENP1 to enhance c-Jun-dependent transcription because SENP1 can desumoylate the CRD1 domain of p300, thereby releasing the cis-repression of CRD1 on p300, Transfection of p300 into prostate cancer cells specifically increases mRNA and protein levels of nuclear matrix peptides lamins A and C., We extended the search for mutations to the EP300 gene and showed that mutations in EP300 also cause this disorder. These are the first mutations identified in EP300 for a congenital disorder., A novel and unique PAX8 mutation provides evidence for a crucial role of the p300 coactivator in mediating the functional synergism between PAX8 and TTF-1 in thyroid-specific gene expression., TCF4 expression mediated by beta-catenin/p300 may be important for initial steps during trans-differentiation of endometrial carcinoma cells., p300 directly acetylates lysines in the carboxyl-terminal region of Foxo1 in vivo and in vitro, and potently stimulates Foxo1-induced transcription of IGF-binding protein-1., We report a novel, noncompetitive mechanism linking acetylation and ubiquitination, in which the association of transcription factor E2F-1 with the cellular coactivator and acetyltransferase p300 determines its acetylation and subsequent ubiquitination., data indicate that p12I appears to play a vital role in HTLV-1-mediated T cell activation by activating calcium-mediated transcription and augmenting the amounts of p300 within T lymphocytes, cyclin D1 plays an important role in cellular proliferation and differentiation through regulation of p300., Histone acetyltransferase activity of p300 is required for transcriptional repression by PZLF., p300 regulates p63-dependent transcription of p21, Sox9 and p300 interact with chromatin and activates transcription via regulation of chromatin modification, both p300 and CBP increase ATF4 transcriptional activity, point mutations are uncommon in patients with myeloproliferative disorders, GABPA and EP300 are essential components of a retinoic acid-induced enhanceosome in myeloid cells., In cells transfected with both IRF-2 and p300/CBP-associated factor , IRF-2 associated with endogenous nucleolin., loss of p300 in HCT116 cells results in potentially aggressive phenotype characterized by reduced adhesion and increased migration, Data shpw that SII is a major component of chromatin transcription and strongly synergizes with p300 (histone acetylation) at a step subsequent to preinitiation complex formation., Data show that nuclear accumulations of p53 and Mdm2 are accompanied by reductions in c-Abl and p300 in zinc-depleted human hepatoblastoma cells., Role for p300 in promoting cell survival, which is independent of its acetyltransferase activity and acts at least in part through FGF-1., Tat-mediated activation of the HIV promoter requires the SWI/SNF complex in synergy with the coactivator p300., DEK interacts with histones and exerts a potent inhibitory effect on both p300 and PCAF-mediated histone acetyltransferase activity and transcription., PPARGC1A, PPARGC1B, and EP300 may have roles for familial breast cancer susceptibility, the transcription factor p300 is a potential repressor of telomerase activation and histone acetylation in the context of E6 expression, Levels of expression of CtBP and p300 are critical for the action of SNAIL and ZEB1, which have a pivotal role in levels of epithelial-mesenchymsal transitionin human colon carcinoma., coordinated binding of Zac zinc fingers and C terminus to p300 regulates HAT function by increasing histone and acetyl coenzyme A affinities and catalytic activity, HDAC4 (histone deacetylase 4) and p300, a known HAT (histone acetyltransferase), reversibly controlled the activity of the IL-5 promoter in vivo and in vitro, with a concurrent alteration of histone H3 acetylation status at the promoter regions., Transcriptional coactivator p300 may regulate fibronectin expression via PARP and NF-kappaB activation in diabetes., hypothesized that HSV-1 ICP0 recruits activated IRF-3 & CBP/p300 to nuclear structures, away from host chromatin; this leads to inactivation & accelerated degradation of IRF-3, resulting in reduced transcription of IFN-beta & inhibition of host response, The patients with EP300 mutations displayed the typical facial gestalt and malformation pattern compatible with the diagnosis of Rubinstein-Taybi syndrome., p72 RNA helicase may not only be involved in the p53-Mdm2 regulatory loop, but also profoundly impact on the transcriptome through various CBP/p300 and P/CAF interacting proteins., MITF evokes transcription of a paradigmatic MITF target tyrosinase and show that the adenoviral E1A protein represses the MITF-driven transcription in these cells., Role for p300 acetyltransferase includes the modulation of chromatin structure and function during DNA metabolic events as well as for transcription., EP300 mutations could be underdiagnosed because they may result in phenotypes different from classical Rubinstein-Taybi syndrome., Asparagine hydroxylation and S-nitrosylation of HIF-1alpha decrease p300 binding, while its phosphorylation does not affect p300 binding, which was reconfirmed by competitive inhibition analyses using mutant peptides., p300-mediated acetylation of p53 requires Bat3, CBP/p300 has an evolutionarily conserved role as a buffer regulating TCF-beta-cat/Arm binding., A complex between tetrameric p53 and p300 in which four domains of p300 wrap around the four transactivation domains of p53., These results indicate that impaired competition with p300 is the possible cause of dysfunction of c-Ski/SnoN in scleroderma fibroblasts and that this might contribute to maintenance of the autocrine TGFbeta loop in this disease., ERK2-mediated C-terminal serine phosphorylation of p300 was a key event in the regulation of EGF-induced keratin 16 expression., These results provide evidence for a model in which Tax and pCREB bind distinct surfaces of KIX for effective CBP/p300 recruitment to the HTLV-1 promoter., demonstrate the distinct regulatory mechanisms of p300 and TR3 on retinoid X receptor-alpha acetylation, Data show that there are combinatorial effects of CBP/p300, Ets2, and PKA triggered via growth factors released from maternal endometrium., These data reveal that phosphorylation of CREB is not sufficient for CBP/p300 recruitment and transcriptional activation., PGE(2) via EP(2) and EP(4) activates the cAMP-->PKA-->CREB pathway leading to enhanced CYP19 transcription and increased aromatase activity through BRCA1 and p300, that Skp2 controls p300-p53 signaling pathways in cancer cells, making Skp2 a potential molecular target for cancer therapy., p300/HAT activation has a role as a very early response to shear that is essential for increasing eNOS mRNA levels, p300 can serve as a scaffold for the E1A repression domain to access specific cellular gene promoters involved in growth regulation., high levels of p300 and C/EBPalpha in suprabasal layers of HPV16 positive cervical lesions allow the viral E2 protein to activate HPV16 early gene expression, C-terminal region of p300 provides corepressor function and facilitates the recruitment of p300 and HDAC3 to the YY1-binding site and represses the c-Myc promoter., A long unstructured chain (or "flap") overlaying the acetyl-CoA binding groove, and a four-alpha-helix "tower" excursion from the main beta-sheet--critically contribute to the recognition and presumptive catalytic machinery of p300., Results identify TFIIIC as a recruitment factor for p300 and thus may have important implications for the emerging concept that tRNA genes or TFIIIC binding sites act as chromatin barriers to prohibit spreading of silenced heterochromatin domains., These observations demonstrate that p300 can inactivate Sirt2 by acetylation and that p300 may regulate the activity of p53 indirectly through Sirt2 in addition to its direct modification of p53., lower transactivity of PPAR delta for arachidonic acid in Caco-2 cells, compared with PPAR alpha, is associated with the binding activity of p300 to the receptor, Fbx3 formed SCF(Fbx3) ubiquitin ligase and promoted the degradation of HIPK2 and p300 by the ubiquitin-proteasome pathway; PML inhibited this degradation, p300 undergoes a dynamic cycle of autoacetylation and deacetylation regulated by SIRT2., Brucella controls host defenses by impairing macrophage responses to IFNgamma induced STAT1-CBP/P300 association., functional inactivation of HIF-alpha by stimulation of FIH-dependent p300 contributes to the YC-1-induced deregulation of hypoxia-induced genes, p300 histone acetyltransferase, one of the NFkappaB-associated coactivators, reactivates epigenetically silenced MLH1 in 293T cells., The effects of specific p53 phosphorylations on its interaction with the Taz2 domain of p300, are examined., Estrogen increases transcription in a rapid but transient manner at early estrogen-repressed genes but that this is followed by recruitment of the corepressor CtBP1, a p300-interacting partner that plays an essential role in the repressive process., The authors demonstrate that the nuclei of Tax-expressing cells, including HTLV-1 transformed T-lymphocytes, contain a pool of Tax molecules acetylated on lysine residue at amino acid position 346 by the transcriptional coactivator p300., STAT3 transactivates its target genes by the recruitment of CBP/p300 co-activators and this process generally does not require the contribution of SRC-1., Thermodynamic experiments revealed the importance of hydrophobic interactions in the complex of Taz2 with p53 phosphorylated at Ser(15) and Thr(18)., demonstrate that the histone acetyl transferase CBP (also known as Nejire) in flies and CBP and p300 (Ep300) in humans acetylate H3K56, Data show that acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 and that acetylation functions in concert with Keap1-mediated ubiquitination in modulating the Nrf2-dependent antioxidant response., p21(WAF1)-p300-DNMT1 pathway may play a pivotal role to ensure regulated DNMT1 expression and DNA methylation in mammalian cell division., NF-kappaB and p300 are involved in the expression of vascular cell adhesion molecule-1 induced by interleukin-1beta in human tracheal smooth muscle cells., p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization., These results point to an important role for p300 in maintaining genomic integrity by negatively regulating c-myc., p300 can inactivate HDAC6 by acetylation; p300 may regulate the activity of Sp1 indirectly through HDAC6 in addition to its direct modification of Sp1., results indicate that PPAR-gamma blocked Smad-mediated transcriptional responses by preventing p300 recruitment and histone H4 hyperacetylation, resulting in the inhibition of TGF-beta-induced collagen gene expression, The authors show that ORF3-mediated extracellularly regulated kinase (Erk) activation was responsible for the observed increase in phosphorylation and transactivation activity of p300/CBP., Oveexpression of p300 upregulated hPTTG at the levels of promoter activity, mRNA and protein expression., epigenetic alterations with upregulated EP300 targeting miRNAs miR-194, miR-200b, miR-200c and miR-429 are related to reduced EP300 mRNA and protein in pancreatic ductal adenocarcinoma, RTK1 specifically inhibits the p300-mediated acetylation of p53 but not the acetylation by another acetyltransferase, p300/CREB-binding protein -associated factor, SENP3 is a redox sensor that regulates HIF-1 transcriptional activity under oxidative stress through the de-SUMOylation of p300, Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3., Data show that cytoplasmic p300/CBP E4 activities ubiquitinate and destabilize p53, while physically separate nuclear p300/CBP activities, such as p53 acetylation, activate p53., Data support a model in which Notch-1 can activate the transcription of ERalpha-target genes via IKKalpha-dependent cooperative chromatin recruitment of Notch-CSL-MAML1 and ERalpha, which promotes the recruitment of p300., CBP is the coactivator directly interacting with HIF-1 alpha N-TAD and mediating the transactivation function of this domain in hypoxia, These findings suggest that p300 is likely to play an important role in biological processes in which transcriptional bookmarking or preconditioning influences cellular growth and development., PGI(2) exerts anti-inflammatory action by inhibiting STAT1-induced cytokine production, in part by targeting the transactivation domain-induced recruitment of the histone acetylase CBP/p300., structure of p300 comprises an atypical helical bundle stabilized by three Zn ions and closely resembles the solution structures determined previously for shorter peptides., propose that individuals with EP300 mutations may exhibit a slightly different phenotype compared to individuals with CREBBP mutations, Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates., These results suggest that in human tracheal smooth muscle cells, activation of MAPKs, NF-kappaB, and p300 are essential for IL-1beta-induced cPLA(2) expression and PGE(2) secretion., Cdk9 forms a functional complex with the p300/GATA4 and is required for p300/GATA4- transcriptional pathway during cardiomyocyte hypertrophy, Tax binds the cyclin D1 promoter-proximal cyclic AMP response element (CRE) in the presence of phosphorylated CREB (pCREB) in vitro, and together the Tax-pCREB complex recruits the cellular co-activator p300 to the promoter., p300 plays a central coactivator role in hypoxic induction of EPO, ). miR-132 regulates innate antiviral immunity by inhibiting expression of the p300 transcriptional co-activator; p300 regulates miR-132 levels, revealing a dynamic equilibrium between miR-132 and p300, p300/HDAC1-induced dynamic changes in the acetylation of Nur77 protein play an important role in the control of Nur77 protein turnover., MAGE-11 links NH(2)-terminal domains of AR and p300 to promote transcriptional synergy through a cadre of FXXLF-related interacting motifs, These data demonstrate that ATM transduces a DNA damage signal to p300, and that ATM-dependent phosphorylation of p300 is required for stabilization of NBS1 proteins in response to DNA damage., The bromodomain is important for p300 to maintain the basal level of histone acetylation and to induce the transcriptional activation of p300-dependent genes., p300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammals., Using a patient-derived cell line, the authors show that p300 sequestration into the chromatin-bound BRD4-NUT fusion protein foci is the principal oncogenic mechanism leading to p53 inactivation., Sp1-mediated RASSF2A gene transcription is activated by p300 through histone acetylation, and this activation plays an important role in inducing late apoptosis., Exon coverage microarray platform detected intragenic deletions, Data show that activator-dependent p300 histone acetylation disrupted both inter- and intrafiber nucleosome-nucleosome interactions and simultaneously led to enhanced RNAPII transcription from the decondensed model chromatin., Our results reveal an essential role of p300-mediated acetylation in regulating the stability of ThPOK, p300 and CBP are implicated in regulation of ER-beta activity and cellular migration in prostate cancer., BCL6 repression of EP300 in human diffuse large B cell lymphoma cells provides a basis for rational combinatorial therapy., Deletion of CBP/p300 specifically and dramatically reduces acetylations on H3K18 and H3K27., transcriptional coactivator p300 may be partially degraded during SMC differentiation, leaving an activated subpopulation with increased HAT activity and SMC differentiation-gene specificity, NF-kappaB-mediated inflammatory signaling is able to block HIF-1alpha transactivation at hypoxia response elements-encoding genes by direct competition for p300 binding at the promoter., Ep300 overexpression is an independent biomarker for poor prognosis of patients with hepatocellular carcinoma., Data show that knock down of p300DeltaC in RC-K8 cells reduced their growth in both liquid culture and soft agar., Recruitment of BRCA1 to the p53-binding region of the p21 promoter in response to DNA damage required methylation of Arg 754 of p300 by CARM1, Cigarette smoke extract regulates cytosolic phospholipase A2 expression via NADPH oxidase/MAPKs/AP-1 and p300 in human tracheal smooth muscle cells., findings show that the transcriptional co-activator p300 was expressed in glioblastoma multiforme tumors and cell lines and acted as an activator of the GFAP gene and a repressor of the Nestin gene, p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity, results identify CREBBP/EP300 mutations as a major pathogenetic mechanism shared by common forms of B-cell non-Hodgkin's lymphoma, with direct implications for the use of drugs targeting acetylation/deacetylation mechanisms, Single Nucleotide Polymorphisms in EP300 is associated with childhood acute lymphoblastic leukemia., Apoptosis Stimulating Proteins of p53 (ASPP) proteins can function together with p300 in regulating the transcriptional activity of p53, High expression of EP300 protein human, suggest poor prognosis for patients with resectable esophageal squamous cell carcinoma., ERbeta and its co-regulators p300 and NCoR are expressed in human transitional cell bladder cancer, Adenosine monophosphate-activated protein kinase (AMPK) inhibits transforming growth factor (TGF)-beta-induced fibrogenic property of activated hepatic stellate cells by regulating transcriptional coactivator p300., Knocking down of p300 expression in hepatoma cells recovered E-cadherin expression, inhibited the translocation of beta (beta)-catenin into the nuclei, decreased cyclin D1 activity and suppressed the migration/invasion of HCC cells., study found AML1-ETO is acetylated by the transcriptional coactivator p300 in leukemia cells isolated from t(8;21) AML patients, and this acetylation is essential for its self-renewal-promoting effects in cord blood CD34(+) cells and its leukemogenicity, CBP/p300 and SIRT1 were recruited to histone gene promoters in an NPAT-dependent manner, knockdown of which affected histone acetylation on histone gene promoters and histone gene transcription., These fi ndings assign a central role for the ATF5/p300 complex in ATF5 function., The authors have identified the acetyltransferase p300 as an additional target of Escherichia coli type III secreted protease NleC., Results indicate that quercetin suppresses COX-2 expression by inhibiting the p300 signaling and blocking the binding of multiple transactivators to COX-2 promoter., Beta-HPV 5 and 8 E6 degrade p300 in a proteasomal-dependent, E6AP-independent manner. E6 competes with AKT for binding to p300., p300 acetylates Notch1 ICD in cell culture assay and in vitro, and conserved lysines located within the Notch C-terminal nuclear localization signal are essential for Notch acetylation., Transcriptional repression mediated by IER5 regulates Cdc25B expression levels via the release of NF-YB and p300 in acute myeloid leukemia., CREBBP and EP300 mutations are not major pathogenetic mechanisms of myeloproliferative neoplasms, The difference in the spatiotemporal expression of p300 and CBP indicated that they play different roles in tooth development., our findings show that the E2A proteins are acetylated at specific residues as a consequence of lysine acetyltranferases(CBP, p300 and PCAF ) recruitment, Evaluated the influence of gene variant A>G rs20551 in EP300 in donor and/or recipient on clinical results after HLA-identical sibling allo-SCT., A hitherto unreported but paternally inherited heterozygous sequence alteration, c.506 1+9C>T in IVS 30-31, which most likely represents a normal variant (NetGene 2 splice prediction software)., p300 and PCAF cooperate in the control of microRNA 200c/141 transcription and epithelial characteristics, We present the first evidence that the portion of E2A proteins found within the oncogenic fusion protein E2A-PBX1 and including the transcriptional activation domains can enhance the HAT activity of CBP/p300, Low expression of p300 is an independent prognostic marker of better survival in operable NSCLC patients., Data show that the interaction between FOXO3a and CBP/p300 is indicated by additional binding sites on CBP/p300., Overexpression of transcription factors CBP/p300 in conjunction with E2A results in enhancement of E2A-induced histone acetylation, germline transcription, and immunoglobulin (Ig)kappa light chain rearrangement., variants of the transcriptional coactivator genes (EP300 and PCAF) may influence Hepatocellular carcinoma risk in populations with low mutations or chromosomal instability rates., Studies provide a mechanism whereby acetylation of Skp2 by p300 regulates protein stability and cellular localization, thus modulating oncogenic signaling., A genome-wide association study on a southern European population identified a new Crohn's disease susceptibility locus at RBX1-EP300., studies suggest that the HPV16-E7 and Ad5-E1A viral oncoproteins bind to the same p300 CH1 domain to disrupt p300 function by distinct mechanisms., GR and p300 are involved in the induction of H6PD by cortisol in human amnion fibroblasts, the intrinsically disordered region of the acetyltransferase p300 with similarity to prion-like domains plays a role in aggregation, A novel upstream role for miR-150 in p300-mediated cardiomyocyte hypertrophy., Data indicate that mutation of E3 ligase motif not only abolishes BRMS1-induced p300 polyubiquitination and degradation, but importantly, dramatically reduces the metastasis suppressor function of BRMS1., CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes., To best of our knowledge, we provide first evidence that GNPs potentiate cell death by activating p300/CBP histone acetyltransferases., p300 associates with the RLIP76 promoter via an overlapping cMYB and cETS binding site and regulates RLIP76 promoter activity and its expression., a stress p53-Mdm2-p300-HDAC1 regulatory network that is activated and stabilised by two regulatory proteins, p300 and HDAC1, is reported., cAMP signalling decreases the level of the p300 protein by promoting its ubiquitin-proteasome dependent degradation, which is mediated by Epac and p38 MAPK, TTC5 and EP300 cooperate to prevent excessive accumulation of MYC in AML cells and their sensitization to cell death., Data indicate that IKKbeta inhibited the binding between TAp63gamma and p300, a co-activator of TAp63gamma, and consequently counteracted the positive effect of p300 on TAp63gamma transcriptional activity., Data indicate that menadione (MD) and ethacrynic acid (EA) decrease expression of luciferase under the control of hypoxia-responsive elements in hypoxic cells as well as to efficiently block the interaction between the full-length HIF-1alpha and p300., Levels of p300, PKCbeta2 and ROS in HG and AGEs groups were elevated by 1.04, 1.26, 0.78 and 1.45, 1.07, 0.71 folds of those in NG group., p300 contributed to both IFN-gamma- and IRF-1-mediated TRIM22 transcription independent of its histone acetyltransferase activity, however, it was required for the recruitment of RNA polymerase II to TRIM22 promoter region., Frameshift mutations of tumor suppressor gene EP300 occur in gastric and colorectal cancers with high microsatellite instability., Charge density distribution and the electrostatic moments of CTPB in the active site of p300 enzyme, CaMKK/Akt/p300 cascade plays an important role in laminar stress-dependent induction of KCa2.3 and KCa3.1 expression, thereby regulating EC function and adaptation to hemodynamic changes., S100A6 and the TAZ2 domain of p300 bind p53 with similar affinities and S100A6 effectively competes with TAZ2 for binding to p53., These results establish a mechanism in which SET1 complex and p300 act cooperatively, through direct interactions and coupled histone modifications, to facilitate the function of p53., Authors identified acetyltransferase p300 and deacetylase HDAC7 as enzymes modulating human T cell leukemia virus type 1 Tax protein acetylation., Present the 2.8-A crystal structure of the catalytic core of p300 containing its bromodomain, CH2 region and HAT domain. The structure reveals that the CH2 region contains a discontinuous PHD domain interrupted by a RING domain., we explored how these HIF-specific hydroxylases contribute to the HIF-1alpha-p300 interaction by FRET measurements along with HIF-1 mediated transcriptional activation, transcriptional associations with HIF-1alpha and NF-kappaB, as well as beta-catenin/p300 complexes, may contribute to modulation of changes in tumor cell kinetics in response to a hypoxic condition in endometrial carcinoma cells, these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation., Data suggest that megakaryoblastic leukemia 1 (MKL1) and histone acetyltransferase p300 could augment the expression of catechol-O-methyltransferase (COMT), increase estrogen metabolism, and thus reduce MCF-7 cell proliferation stimulated by estrogen., Data suggest that SMYD2-mediated estrogen receptor alpha (ERalpha) protein methylation and p300/cAMP response element-binding protein-binding protein-dependent ERalpha acetylation play an important role in the estrogen-induced gene expression profiles., Immunohistochemical p300 expression is significantly associated with histology and lymph node involvement in colorectal cancer, and overexpression may be an independent favorable prognostic factor for disease-free survival., P300 drives an angiogenic transcription program during hypertrophy that is fine-tuned in part through direct repression of p300 by miR-20a., Findings demonstrate that CypB prevents hypoxia-induced cell death through modulation of ubiquitin-mediated CHOP protein degradation, suggesting that CypB may have an important role in the tight regulation of CHOP under hypoxia., 25 cluster by targeting a transcriptional activator of E-cadherin., Sp1 may regulate the expression of CD59, whereas CREB-binding protein (CBP)/p300 bridge NF-kappaB and CREB, which functions as an enhancer-binding protein to induce the up-regulation of CD59 during in lipopolysaccharide (LPS)-triggered complement activation., results reveal a novel post-translational regulation of MTA2 by the way of p300-dependent acetylation, which is important for tumor cells growth and migration and provides a potential target for clinical cancer research, KAT3B acetyltransferase activity inhibition by 1,4-naphthoquinones involves redox cycling and nucleophilic adduct formation, this study indicates that EP300-related Rubinstein-Taybi syndrome cases occur more frequently than previously thought ( 8% vs 3-5%), Phosphorylation of p300 increases its protein degradation to enhance the lung cancer progression., The combination of p300 and CBP expression may be useful in identifying patients with increased risks of cancer recurrence of small cell lung cancer., Based on analysis, HSF1 activation upon heat shock involves the function of multiple chromatin modifiers, centering on the EP300/CREBBP histone acetyltransferase complex. Silencing of EP300 destabilizes HSF1 in nonstressed cells and induces its proteasomal degradation., A complex comprising p300/c-Jun/PARP-1 that bound wild type., Factor acetyltransferase p300 (FATp300), a major epigenetic regulator that acetylates specific lysines in histones and transcription factors, is essential for elevated collagen synthesis and the levels of FATp300 are significantly elevated in different fibrotic tissues., EDAG forms complex with GATA1 and p300 and increases GATA1 acetylation and transcriptional activity by facilitating the interaction between GATA1 and p300, Our results indicate a cross-talk between Braf and p300 in melanoma and demonstrate the importance Braf and p300 expression in the diagnosis and prognosis of melanoma., Promoter-bound p300 complexes facilitate post-mitotic transmission of transcriptional memory., Results show that p300 plays an important role in regulating the expression of key pluripotency genes in dental pulp cells and modifying odontogenic differentiation., Study tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternative EDB exon; these data suggest that histone acetylation could be one of the mechanisms how promoter and promoter binding proteins influence alternative splicing., estrogen receptor binding and p300 redistribution have a role in a squelching mechanism for estradiol-repressed genes, p300 overexpression and its association with clinical factors in patients with OSCC, it is likely that p300 itself or one of its target genes plays a key role in the aggressive phenotypes of OSCC., Data indicate that eletion of the PHD finger impairs histone acetylation by p300 protein., the presence of p300 in the cytoplasm correlates with increased survival of breast cancer patients, MiR-630 reduced apoptosis by downregulating several apoptotic modulators, PARP3, DDIT4, and EP300., P28(GANK) interacted with p300 to attenuate assembly of RelA with p300., this work has broad implications beyond myeloma because shed syndecan-1 is present in high levels in many tumor types as well as in other disease states., Data indicate that HPV16E7, CBP/p300, and retinoblastoma protein pRb interactions are potentially important for cellular transformation., destabilization of p300/CBP by downregulation of iASPP expression levels appears to represent a molecular mechanism that contributes to chemoresistance in melanoma cells., Our patient expands the phenotypic spectrum in EP300-related Rubinstein-Taybi syndrome; this case demonstrates the evolving practice of clinical genomics related to increasing availability of genomic sequencing methods., ER-alpha, SRC-3, and p300 form an active protein complex on estrogen response element-DNA., Data show that Naphthol AS-E phosphate inhibits proto-oncogene protein Myb activity by disrupting its interaction with E1A binding protein p300., MRTF-A and p300 activated the transcription of VE-cadherin gene by enhancing acetylation of histones., ZBRK1 suppresses renal cancer progression perhaps by regulating VHL expression through formation of a complex with VHL and p300 in renal cancer, Data from studies using peptide fragments suggest that complexes formed by the two transcriptional subdomains of TP53 (tumor protein p53) with EP300 exhibit different conformations and play critical roles in p53 transcriptional activity., Results show that the coactivator p300 has both crotonyltransferase and acetyltransferase activities, and that p300-catalyzed histone crotonylation directly stimulates transcription to a greater degree than histone acetylation., DDX24 inhibits p300 mediated acetylation of p53., these data suggest that CBP/p300 are promising therapeutic targets across multiple subtypes in acute myeloid leukemia., Our results link FGF8, c-Abl and p300 in a regulatory pathway that controls DeltaNp63alpha protein stability and transcriptional activity., the histone methyltransferase core enzyme ASH2L was bound at EGFR in the germinal matrix and in gliomas where levels of H3K4me3 are high, and the histone acetyltransferase P300 was bound in samples with H3K27ac enrichment, The Tax oncogene enhances ELL incorporation into p300 and P-TEFb containing protein complexes to activate transcription., DOT1L epigenetically derepresses epithelial-mesenchymal transition transcription factors with c-Myc and p300., levels of p300 protein are temporally maintained in ligand-enhanced skeletal myocyte development. this maintenance of p300 protein is observed at the stage of myoblast differentiation, which coincides with an increase in Akt phosphorylation., p300 and MRTF-A synergistically enhance the expression of migration-related genes in breast cancer cells., these results demonstrate that an interaction between STAT1, p300, and peroxisome proliferator-activated receptor-gamma is required for 15(S)-HETE-induced CD36 expression, oxidized low density lipoprotein uptake, and foam cell formation, RORgammat is acetylated, and this acetylation is reciprocally regulated by the histone acetyltransferase p300 and the histone deacetylase HDAC1., coactivator p300 mediates cytokine-induced hiNOS transactivation by forming a distant DNA loop between its enhancer and core promoter region, Sox4 was also able to promote b-catenin-mediated transcription of the Slug gene through formation of transcriptional complexes with b-catenin and p300, independent of TCF4 status, PIAS1 enhances p300 recruitment to c-Myb-bound sites through interaction with both proteins. In addition, the E3 activity of PIAS1 enhances further its coactivation,
OMIM_DISEASE Colorectal cancer, somatic, Rubinstein-Taybi syndrome 2,
SP_COMMENT catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone., disease:Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3., disease:Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies., disease:Defects in EP300 may play a role in epithelial cancer., function:Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes., online information:P300/CBP entry, PTM:Acetylated on Lys at up to 17 positions by intermolecular autocatalysis., PTM:Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1., PTM:Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1., PTM:Phosphorylated., similarity:Contains 1 bromo domain., similarity:Contains 1 KIX domain., similarity:Contains 1 ZZ-type zinc finger., similarity:Contains 2 TAZ-type zinc fingers., subunit:Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, PRDM6, SPIB, SRY, TCF7L2, TP53, SRCAP, TTC5, JMY and TRERF1. The TAZ-type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with ING5. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat.,